不同灭菌方法对人牙周膜成纤维细胞培养的研究.pdfVIP

2008年1月第3卷第1期 China medicine ． 论 著 ． 不同灭菌方法对人牙周膜成纤维细胞培养的研究 李霞 任娟 孙克勤 程珏 葛学军 王翔宇 【摘要】 目的 比较2种不同的灭菌方法对人牙周膜成纤维细胞原代培养成活率的影响，寻找较为合 适的灭菌方法，旨在提高人牙周膜成纤维细胞原代培养的成活率。方法 28例牙齿标本分为5倍抗生素溶 液灭菌组15例和次氯酸钠溶液灭菌组13例，2组分别采用5倍抗生素溶液与次氯酸钠溶液对取得的人牙周 膜组织进行灭菌后，均采用组织块法培养人牙周膜成纤维细胞，并对细胞进行形态学和免疫组织化学染色鉴 定，比较2种不同灭菌方法细胞培养的成活率。结果 次氯酸钠溶液灭菌组无l例细胞成功传代，5倍抗生 素溶液灭茵组细胞培养成活率为46．7％，且符合人牙周膜成纤维细胞的形态学和免疫学特征。结论 利用 舍5倍抗生素溶液灭菌效果与次氯酸钠溶液无差别，但相对更利于人牙周膜成纤维细胞的成活。 【关键词】 人牙周膜成纤维细胞；灭菌； 原代培养 【中图分类号】 R 78 【文献标识码】A 【文章编号】 1673-4777(2008)01-0010-02 The influence of different ste i面ng methods on the culture of human periodontal ligament fibroblasts Li Xia，Ren Juan，Sun Keqin，Cheng Jue，Ge Xuejun，Wang Xiangyu．Department of Endodontics，College of Stomatology，Shanxi Medical University(Taiyuan 03O0o1， ina) 【Abstract】 Objective To compare the influence of two different sterilizing methods on the primary culture of human periodontal ligament fibroblasts(HPLFS)．Methods One group used sodium hypochlorite solution and 8fflother used isotonic Na chloride with 5× antibiotics to sterilize human periodontal Iigament．then human periodontal ligament fibroblasts were cultured by tissue explant culture technique． Morphological and immunocytochemieal analysis wefe used to characterize the cell lineage．The achievement ratio of the two methods Was compared．Results In the sodium hypochlorite solution group，none of the cells had successful passage．In the isotonie Na chloride with 5× antibiotics group。the culture achievement ratio Was 46．7％。and the cells morphological and immunocytochemical characteristics analyzed by tissue explant culture technique wel_e consistent with those of typical HPLFs．Conclusion The sterilizing effect of sodium hypocbiotite solution and isotonic Na chloride with 5×antibiotiCS had no difference．But isotonic Na chloride with 5×antibiotic