重组乙酰胆碱酯酶的表达及其分子进化研究进展.pdf

董洁娴 等/重组乙酰胆碱酯酶的表达及其分子进化研究进展 557 生 物 工 程 学 报 Chinese Journal of Biotechnology May 25, 2012, 28(5): 557−564 /cjbcn ©2012 Chin J Biotech, All rights reserved 综 述 董洁娴，李振峰，雷红涛，何永盛，王弘，孙远明 华南农业大学食品学院 广东省食品质量安全重点实验室，广东 广州 510640 董洁娴, 李振峰, 雷红涛, 等. 重组乙酰胆碱酯酶的表达及其分子进化研究进展. 生物工程学报, 2012, 28(5): 557−564. Dong JX, Li ZF, Lei HT, et al. Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review. Chin J Biotech, 2012, 28(5): 557−564. 摘 要: 乙酰胆碱酯酶 (Acetylcholinesterase ，AChE) 是酶抑制法检测农药残留的核心试剂。但天然提取的 AChE 存在含量低、纯化困难、稳定性差等不足，因此，高性能的重组AChE 制备成为研究学者关注的热点。 文中综述了重组AChE 的表达、和分子进化等方面的研究成果，并指出酶定向进化策略与表面展示技术结合是 重组AChE 活性改造的未来趋势。 关键词: 农药残留，重组乙酰胆碱酯酶，酶抑制检测法 Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review Jiexian Dong, Zhenfeng Li, Hongtao Lei, Yongsheng He, Hong Wang, and Yuanming Sun Guangdong Provincial Key Laboratory of Food Quality and Safety, Food College of South China Agricultural University, Guangzhou 510640, Guangdong, China Abstract: Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Received: August 30, 2011; Accepted: