压片法检查蓝藻液泡 - 植物科学学报.pdf

2001, 19(5) : 416420 J ournal of Wuha n Botanica l Resear ch 1 2 2 郭厚良　赵以军　吴红艳 ( 1. , 430072; 2. , 430079) : 7120( . 7120) 0. 1 / 4 ,90% A nabaena sp PCC mol L NaCl d , 1, , , , , , , : ; ; :Q949.22: A: 1000-470 ( 2001) 05-0416-05 Determination of Vacuoles from the Blue-green Algae by Squash Method 1 2 2 GUO Hou-Liang , ZHAO Yi-Jun , WU Hong-Yan ( 1. College of Lif e Sciences W uhan University Wuhan, , 430072, China; 2. College of Lif e Sciences, Central China Normal University, Wuhan430079, China) Abstract: The blue-green algaA nabaena sp. PCC 7120 was grown in the medi- 0. 1 / 4 90% - um containing mol L NaCl for d and over of cells became vacuola ted If they were grown in the common condition for over month only a part. , of cells began vacuolation The cultures were dropped on slide and a filter pa-. per was put on the cover slip to absorb the surplus water A light pressure on. the cover slip was made by a thumb to break some vacuolated cells. Some vac- uoles were released from the vacuolated cells. The vacuoles were fully trans- parent, varied in size, they have not be showed under general microscope but under phase contrast microscope. Two kinds of cell vacuolations by salt induc- ing or by aging have parallelism. : - ; ; Key words Blue green algae Vacuoles Squash method 20 : 2001-01-02, : 2001-05-10 : ( : : ( 1940- ) ,, , 5 :