生物节律紊乱对小鼠脂代谢的影响.PDFVIP

二、结题报告 生物节律紊乱对小鼠脂代谢的影响 上海医学院 迪娜·索力提肯 王娜 指导老师：钱睿哲 摘要：机体的生理活动、生化代谢、内分泌功能、行为改变等均表现昼夜节律现象。 现目前的研究表明脂代谢还受到生物钟基因的部分影响，脂代谢的基因的表达与生物钟基因 的表达呈相关性且会随着生物钟基因表达的起伏而变化。为了探讨小鼠体内生物钟基因以及 脂代谢基因表达节律关系，观察光照周期对于脂代谢产生的影响。本实验通过复制“昼夜节 律紊乱的小鼠”模型，使用Realtime-PCR 及生化检查的方法观察小鼠体内钟基因和脂基因 的节律变化以及血脂血糖的变化。实验显示3 个脂代谢基因的表达节律与4 个生物钟基因的 表达节律呈相关性，脂代谢的基因随着生物钟基因表达的起伏而变化。揭示光照可能对脂代 谢节律产生一定的影响作用。 关键词：生物节律 生物钟基因 脂代谢基因 昼夜节律 Abstract: The bodys physiological activity, metabolism and endocrine function, behaviour, all above show the phenomenon of circadian rhythm. The current study is that lipid metabolism is also partially affected by some of circadian clock genes, lipid metabolism gene expression was related with the circadian clock gene expression. When circadian clock genes expression change, lipid metabolism genes expression will also change.To investigate the relationship between the biological clock genes and lipid metabolism genes expression rhythms in mice, we have observed the cycle for the impact of lipid metabolism. In this study, by copying the model of circadian rhythm disordered mice, using the Realtime-PCR to check the rhythm change of the clock genes and the lipids genes in mice, using biochemical examination to check the blood lipid and blood glucose. Experiments shows that 3 lipid metabolism genes expression rhythm is correlated with 4 clock genes expression rhythm, when circadian clock genes expression change up and down, lipid metabolism genes expression will change. We reveal the illumination rhythm may have some influence on metabolism rhythm. Key words: Biological rhythms Clock gene Lipid metabolism gene Circadian rhythms 引言 机体的生理活动、生化代谢、内分泌功能、行为改变等均表现昼夜节律现象。 这种节律的紊乱可导致多种疾病，如代谢失调、肿