植骨气囊在人工关节松动动物模型实验研究中的应用.pdfVIP

· 36· ! ： 笪 查 2o08年6月第2卷第3期 Chin J Joint Surg(Electr0nic Vemi0n)，Jun 2008，Vol 2，N0．3 基 础 论 著 · (诺华扶他林杯征文) 植骨气囊在人工关节松动动物模型实验 研究中的应用 陶毙 沈灏 张先龙 王琦 邵俊杰 彭晓春 【摘要】 目的 建立一种人工关节无菌性松动的实验动物模型并且定量评价磨损颗粒诱导的 骨溶解。方法 在8～l0周大的近交系BALB／c小鼠背部注射无菌空气形成气囊，后将近交系小鼠 颅骨植入气囊。实验组小鼠气囊内注入聚乙烯磨损颗粒，对照组气囊内注人生理盐水，植骨后14 d 处死小鼠，取出气囊组织和颅骨骨片进行炎性因子(IT一1、TNF、VEGF)和破骨细胞激活相关基因 (RANK／RANKI )的分子生物学检测。结果 聚乙烯颗粒刺激组气囊炎性反应较对照组明显，炎性 细胞渗出增多，气囊囊壁变厚；在颗粒刺激组的囊壁和骨组织界面上可以观察到大量抗酒石酸酸性 磷酸酶染色(TRAP)阳性的破骨细胞，HE染色可见骨表明有明显的点状侵蚀，图像软件分析显示两 组问差异均有统计学意义(P0．05)。ELISA法和RT—PCR法分别测量囊壁组织内炎性因子IL一1， TNF，VEGF含量和RANK的mRNA含量，两组问的差异均有统计学意义(P0．05)。结论 植骨气 囊模型是一种经济、快速、有效的模拟人工关节松动病理过程的实验动物模型。 【关键词】 气囊；模型，动物 Bone implant in air pouch，an aseptic loosening animal model TAO Kun，SHEN Hao，ZHANG Xian— long，WANG Qi，SHAO Junjie，PENGXiao—chun．Department ofOrthopaedics，Sixth People’S Hospital of Jiaotong University，Shanghai 200233，China Corresponding author：ZHANG Xian—long．E—mail：=xl40@v／p．sina．COrn 【Abstract】 0bjective To establish an animal model for aseptic loosening of total joint replacement and quantitatively evaluate the wear—debris induced osteolysis．Methods Air pouches were established on the back of BAI B／e mice by injecting sterile air subcutaneously．A section of calvaria from a syngeneic mouse donor was implanted in the air pouch．One group of bone—implanted pouches was stimulated with polyethylene debris，andthe remaining bone—implanted pouches received saline alone as controls．14 days after the implantation，all mice were killed．The air pouches with the implanted calvaria were obtained for molecular and histological analyses．Results Compared with the control group，inflammatory responses， osteoclasto genesis，and bone resorption were much more obvious in the experimental group．The difference between them were significantly(尸0．05)．IL一1