茶叶提取物与二氢杨梅素抑菌活性比较研究.pdfVIP

安徽农业大学学报，2008，35(2)：239—242 Journal of Anhui Ag6cultural University 茶叶提取物与二氢杨梅素抑茵活性比较研究 萧力争 ，郭 维 ，刘素纯 ，银 霞 (1．湖南农业大学园艺园林学院，长沙410128；2．湖南农业大学食品科技学院，长沙410128) 摘 要：采用滤纸片法和试管二倍稀释法研究茶叶提取物、二氢杨梅素对细菌、酵母和霉菌的抑菌活性，包括 茶叶提取物、二氢杨梅素的最低抑菌浓度(MIC)以及茶叶提取物与二氢杨梅素的质量浓度、pH值、温度对其抑菌 活性的影响。结果表明，茶叶提取物和二氢杨梅素对细菌均有较强的抑菌活性，对供试酵母和霉菌的抑制效果不 明显。茶叶提取物对细菌的最低抑菌浓度不超过10 g·L-‘，在中性或弱碱性环境中比在强碱性环境中的抑菌活性 强，二氢杨梅素对细菌的最低抑菌浓度不超过1．76 g·L-‘，在酸性或弱碱性环境中比在中性和强碱性环境下的抑 菌活性更强。 关键词 ：茶叶提取物；二氢杨梅素；细菌；酵母；霉菌；抑菌效果 中图分类号：$571．109．9 文献标识码：A 文章编号：1672—352X(2008)02-0239-04 A comparative study on antimicrobial activity of tea-extract and dihydromyricetin XIAO Li—zheng ，GUO Wei ，LIU Su—chun ，YIN Xia (1．College of Horticulture and Landscape，Hunan Agricultural University，Changsha 410128； 2．College of Food Science and Technology，Hunan Agricultural University，Changsha 410128) Abstract：The antimicrobial activities of tea—extract and dihydr0myricetin were studied by filtering paper meth— od and tube double dilution method．The study included the MIC of tea—extract and dihydr0myricetin and influence of other factors，i．e：the concentration，pH，temperature on the antimicrobial activities of tea—extract and dihydro— myricetin．The results showed that tea—extract and dihydromyricetin had strong inhibition on bacteria activities but no distinct inhibition on yeast and mold．The minimum antimicrobial concentration of tea—extract was no more than 1 0 g‘L～，antimicrobial activities in natural pH or light alkali environment were stronger than those in high alkali environment．The minimum anti—microbial concentration of dihydromyricetin was no more than 1．76 g·L一 。anti—mi crobial activities in light alkaline or acidic environment were stronger than those in natural pH and high alkali envi— ronment． Key words：tea—extract；dihydromyricetin；b