脉冲磁场对人卵巢癌skov3细胞内钙离子和 - 第三军医大学学报.doc

脉冲磁场对人卵巢癌SKOV3细胞内钙离子和细胞膜电位的影响龙映妃1 唐均英1 米彦2 储怡道2 芮少琴2 (400016重庆，重庆医科大学附属第一医院妇产科1；400044重庆，重庆大学电气工程学院高电压与电工新技术教育部重点实验室2) 摘要 目的 研究脉冲磁场 (8HZ，200mt)对卵巢癌细胞SKOV3细胞内钙离子和细胞膜电位的影响及其作用机制。方法分为对照组、组、+维拉帕米组。分别用荧光探针Fluo-3/AM及DiBAC4(3)及标记细胞内钙离子和细胞膜电位，应用激光共聚焦显微镜检测磁场处后细胞内平均荧光强度的变化并采用流式细胞检测磁场作用后细胞凋亡情况。结果 脉冲磁场诱导Fluo-3/AM荧光强度增加，与对照组差异有统计学(P0.05)，维拉帕米干预后钙离子荧光强度较组减弱，差异有统计学意义 (P0.05)。脉冲磁场诱导DiBAC4(3)荧光强度增加，细胞膜发生去极化，与对照组比较差异有统计学意义 (P0.05)；维拉帕米对脉冲磁场的去极化效应无明显影响 (P0.05)。 脉冲磁场凋亡率与对照组比较差异无统计学意义 (P0.05)。结论 脉冲磁场作用于SKOV3细胞膜使其发生去极化，从而激活电压依赖型钙离子通道，细胞内钙离子浓度升高。而目前的脉冲磁场实验条件下，未促进细胞凋亡的发生。 关键词脉冲磁场；卵巢癌细胞；细胞膜电位；钙离子 中图分类号 R318.04 [文献标识码]A [通讯作者唐均英 ,tjy168168@, 电话 023基金项目国家自然科学基金 Effects of pulsed electric-magnetic field on intracellular calcium ions and cell membrane potential in human ovarian cancer SKOV3 cells Long Yingfei 1,Tang Junying1,Mi Yan2,Chu Yidao2,Rui Shaoqin2(1Department of Gynecology and Obstetrics,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China; 2State Ministry of Education Key Laboratory of High Voltage and Electrical New Technology,College of Electrial Engineering,Chongqing University,Chongqing 400044,China) [Abstract] Objective: To explore the effect of pulsed electric-magnetic field (8HZ, 200 mt) on the ovarian cancer cell line SKOV3.The effects of PEMF(pulsed electric-magnetic field) on intracellular calcium ions and cell membrane potential were studied. Methods: The cells were divided into three groups: control group, PEMF group, PEMF+verapamil group.The cells in control group did not receive any treatment ,cells in the other two groups were exposed to PEMF 1h, 50umol/L verapamil was given to PEMF+verapamil group. Calcium ions was labeled by fluorescence probe Fluo-3 / AM and membrane potential was labeled by fluorescence probe DiBAC4(3), then laser confocal microscope was utilized to detect the changes of mean fluorescence intensity; Annexin V/PI combined flow cytometry was used to assay apo