流感病毒感染小鼠及病毒载量的变化研究 - 中国实验动物学报.doc

H1N1流感与H7N9禽流感感染BALB/c小鼠发病特点初步研究 杨玉琴1#，徐春华1#，朱召芹1#，田棣1, 陈丽香1，杨华1, 秦波音1，宋志刚1，管文彩1，刘祎1，蔡家麟1，周晓辉1，周文江1,2 （1.上海市公共卫生临床中心，上海，201508；2.复旦大学药学院，上海，201203） [摘要] 目的 本实验旨在初步探讨 A/Shanghai/4664T（H7N9)和A/Puerto Rico/8/34（H1N1）病毒感染BALB/c小鼠的发病特点，为H7N9致病机制与防治的研究提供数据支持。方法 将同等剂量（5×103 TCID50）流感病毒滴鼻感染BALB/c小鼠，分析其在感染后体重、肺指数、病毒载量和肺组织病理的变化情况。结果 H1N1 PR8感染组和H7N9感染组小鼠在7d内体重均持续减低，H1N1 PR8组较H7N9组降低更为明显；在感染后3d H7N9组与PBS组肺指数无显著差异(P＞0.05)，但感染后7d H7N9组与PBS组相比显著增高(P＜0.05)；H1N1 PR8组比H7N9组感染后3d肺指数显著增高(P＜0.05)，但感染后第7d无显著差异 (P＞0.05)。H1N1 PR8组和H7N9组病毒载量在感染后第3天均显著升高，但两组无显著差异；第7天两组病毒载量均有所降低，但H7N9组病毒载量显著高于H1N1 PR8组 (P0.05)。 H1N1 PR8在感染后3d主要病理变化为炎症细胞浸润和水肿，而H7N9组主要轻度炎症细胞浸润；在感染后7天H1N1 PR8组可见大量炎症细胞浸润，出现大面积肺水肿；H7N9组表现为大量炎症细胞浸润。结论 H7N9和H1N1 PR8感染BALB/c小鼠均可发病，但发病特点有所差异，H7N9病毒对鼠的适应性比H1N1 PR8差。 [关键词] H7N9； H1N1 PR8；BALB/c小鼠 [中图分类号] R33 [文献标识码] A [文章编号] YX2013459 A preliminary research on clinical characteristic of BALB/c mice infected by H7N9 and H1N1 PR8 virus Yang Yu-qin1, Xu Chun-hua1, Zhu Zhao-qin1, Tian Di1, Chen Li-xiang1,Yang Hua1, Qin Bo-yin1, Song Zhi-gang1, Guan Wen-cai1, Liu Yi1, Cai Jia-lin1, Zhou Xiao-hui1, Zhou Wen-jiang1,2 Shanghai Clinical Center of Public Health, Shanghai, 201508,China, 2. School of Pharmacy of Fudan University, Shanghai, 201203, China） [Abstract] Objective To compare the clinical characteristic of BALB/c mice infected by H7N9 (A/Shanghai/4664T) or H1N1 PR8 (A/Puerto Rico/8/34) virus for providing clues for H7N9 virus pathogenic mechanismresearch.. Method Each BALB/c mouse was inoculated by 5×103 TCID50 H7N9 or H1N1 PR8 virus or PBS after anesthetize. The bodyweight change, lung index, viral load and pulmonary pathology were monitored and analyzed. Result BALB/c mice infected with H1N1 PR8 and H7N9 showed clinical characteristic differences -In terms of bodyweight change, both H1N1 PR8 group and H7N9 group were reduced gradually within 7 days post infection (d.p.i.), though the bodyweight of H1N1 PR8 group decreased more rapidly than that of H7N9 group. The lung vir