细菌化学趋向性受体聚集体的相互作用 - ingenta connect.pdf

See discussions, stats, and author profiles for this publication at: /publication/265133437 Molecular Interactions of Bacterial Chemoreceptor Assemblies Article in CT PHYSICO-CHIMIC SINIC · July 2014 DOI: 10.3866/PKU.WHXB201404301 CIT TIONS RE DS 0 9 3 authors, including: Luhua Lai Peking University 298 PUBLIC TIONS 6,2 1 CIT TIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Protein design View project ll content following this page was uploaded by Luhua Lai on 27 ugust 2016. The user has requested enhancement of the downloaded file. ll in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately. 物理化学学报(WuliHuaxueXuebao) July ActaPhys. -Chim.Sin.2014,30(7),1347-1353 1347 [Article] doi:10.3866/PKU.WHXB201404301 细菌化学趋向性受体聚集体的相互作用 余大启1,2 涂豫海2,3 来鲁华1,2,* 1 2 ( 北京大学化学与分子工程学院,分子动态与稳态国家重点实验室,北京分子科学国家实验室,北京100871; 北京大学定量 3 生物学中心,北京100871; IBMT.J.WatsonResearchCenter,YorktownHeights,NY10598,USA) 摘要: 细菌化学趋向性受体的最小结构单元为二聚体,在细胞膜上这些二聚体会聚集成大团簇.X射线晶体结 构和低分辨电镜结构测定表明,这些团簇有两类不同的形式,一种是在晶体结构中观察到的倒金字塔式二聚体 的三聚体重复形成的聚集,另一种为由二聚体尾部相互盘绕形成的拉链状聚集.有关拉链状聚集的详细分子模 型目前尚不清楚.本文使用蛋白质-蛋白质对接的方法研究了大肠杆菌丝氨酸化学趋向性受体Tsr二聚体之间 的相互作用.分子对接计算表明,倒金字塔式聚集和拉链状聚集的基本复合物都是可以出现的,相应复合物的 分子动力学模拟表明这些结构都具有一定的稳定性.对于