植物中的蛋白质二硫键异构酶及其类蛋白.pdf

植物生理学报 Plant Physiology Journal 2013, 49 (8): 715~72 1 715 植物中的蛋白质二硫键异构酶及其类蛋白 1 2 2,* 陈珍, 江琼, 朱诚 1台州学院生命科学学院, 浙江台州318000; 2 中国计量学院生命科学学院, 杭州310018 : (protein disulfide isomerase, PDI) , , 摘要 蛋白质二硫键异构酶 及其类蛋白 是硫氧还蛋白超家族的一员 具有催化蛋白质二硫 , , 键氧化、还原和异构的功能 并具有分子伴侣和抗分子伴侣活性及钙离子结合位点 在生物体内起着极其重要的作用。本 PDILs , PDI , 文综述了近年来对植物 的相关了解 总结了 类蛋白的结构、表达、定位、生化与生物学功能 也结合本实验室的 , PDI , 工作 对水稻等 的相关信息与作用做了简要阐述 为加快这一重要酶及分子伴侣的研究提供启示。 : 关键词 蛋白质二硫键异构酶; 分子伴侣; 二硫键氧化与还原; 二硫键异构 Protein Disulfide Isomerise and PDI-Like Proteins in Plant 1 2 2,* CHEN Zhen , JIANG Qiong , ZHU Cheng 1College of Life Sciences, Taizhou University, Taizhou, Zhejiang 318000, China; 2 College of Life Sciences, China Jiliang University, Hangzhou 310018, China Abstract: Protein disulfide isomerase (PDI) and PDI-like proteins (PDILs), belonging to thioredoxin (Trx) su- perfamily, have diverse functions in organisms. They can catalyze thiol-disulfide interchange, resulting in the formation, reduction, or isomerization of protein disulfide bonds in protein substrates. They also display chaper- one activity and anti-chaperone activity. Furthermore, they have Ca2+-binding domain. Researches about PDI and PDILs from plant were summarized in this paper, including structure, expression, location, chemical func- tion and biological role. Related work by our research group about PDILs in rice was also presented. It may bring us a good insight for further research about this enzyme or chaperone with important roles. Key words: protein disulfide isomerase; chaperone; oxidation or reduction of disulfide bonds; isomerization of disulfide bon