白菜转脂蛋白cambp10 分子中钙调素结合结构域的鉴定.pdf

ISSN 1007 -7626 中国生物化学与分子生物学报 http :/ / cjbmb ． bjmu ． edu ． cn 20 11 年 10 月 CN 11-3870 / Q Chinese Journal of Biochemistry and Molecular Biology 27 (10 ):942 ～ 949 白菜转脂蛋白CaMBP10 分子中钙调素结合结构域的鉴定 金犂天， 胖铁良， 李振鹏， 谢万钦， 李翠凤* (南开大学生命科学学院生物化学与分子生物学系，天津 30007 1) 摘要 植物转脂蛋白(LTPs)是多基因编码的蛋白家族，广泛分布于高等植物，其确切的生理功能 至今仍不清楚． 本室从白菜中分离的钙调素结合蛋白-10 (CaMBP10 ) 经序列分析被鉴定为植物转 脂蛋白家族成员，体外实验证明钙调素(CaM )调节其脂质结合活性． 为了深入了解转脂蛋白与 CaM 的相互作用机制，本文通过删除、缺失和定点突变等分子生物学手段确定了白菜转脂蛋白 CaMBP10 分子中的钙调素结合结构域． 该结构域位于分子 C 末端 64 ～ 83 位氨基酸残基之间，其中 疏水氨基酸的分布具有 1-5 -8-10 的CaM 结合模序特征． 关键词 植物转脂蛋白; 钙调素结合蛋白; 钙调素结合结构域; 脂质结合活性 中图分类号 Q74 Identification of CaM -binding Domain of Cabbage Lipid Transfer Protein CaMBP10 * JIN Li-Tian ，PANG Tie-Liang ，LI Zhen-Peng ，XIE Wan-Qin ，LI Cui-Feng (Dep artment of B iochemistry and Molecular B iology ，Nankai University ，Tianj in 30007 1 ，China ) Abstract Plant non-specific lipid transfer proteins (ns-LTPs )encoded by multigene families are distributed ubiquitously throughout the plant kingdom ． Their biological functions in vivo remain unclear ． Recently ，it has been proposed that ns-LTPs may play a key role in plant defense mechanisms ， particularly during the induction of systemic acquired resistance ． However ，very little was known about the regulation in this process． A CaM-binding protein-10 (CaMBP10 )isolated from Chinese cabbage ，is identified as a new member of lipid transfer protein family ． It was found that the lipid-binding activity of CaMBP 10 is