荧光探针量子点检测重金属与有害元素的研究进展及其应用于中药材 .pdf

中草药 Chinese Traditional and Herbal Drugs 第 46 卷 第 18 期 2015 年 9 月 ·2809 · 荧光探针量子点检测重金属与有害元素的研究进展及其应用于中药材安全 性评价的展望 1, 2 1 1 2 1 罗红丽 ，胡一晨 ，骆骄阳 ，万 丽 ，杨美华 1. 中国医学科学院 北京协和医学院药用植物研究所，北京 100193 2. 成都中医药大学药学院，四川 成都 611137 摘 要：近年来，运用新型荧光探针量子点对重金属 Pb （II ）、Cu （II ）、Hg （II ）等及有害元素 As （III ）的检测逐渐成为 科研领域的研究热点。列举了量子点的优越光学性质与运用量子点荧光探针检测重金属与有害元素的优势，阐述了离子络合 反应、电子转移过程、荧光共振能量转移等引起的量子点荧光淬灭、荧光增敏及结合 DNA 滚环扩增、发射波长红移及比率 荧光等方法检测重金属及有害元素的原理，并总结了量子点荧光探针在重金属与有害元素检测中的应用，以期为量子点荧光 探针应用于中药中的重金属与有害元素的快速检测提供参考。 关键词：量子点；重金属；有害元素；中药材；快速检测 中图分类号：R286.015 文献标志码：A 文章编号：0253 - 2670(2015)18 - 2809 - 07 DOI: 10.7501/j.issn.0253-2670.2015.18.024 Research advances on detecting heavy metals and harmful elements based on fluorescent probe quantum dots 1, 2 1 1 2 1 LUO Hong-li , HU Yi-chen , LUO Jiao-yang , WAN Li , YANG Mei-hua 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science Peking Union Medical College, Beijing 100193, China 2. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China Abstract: In recent years, it is a hot spot of research on detecting heavy metals such as Pb (II), Cu (II), and Hg (II) and harmful element As (III) based on novel fluorescence probe quantum dots (QDs). This review introduced the preponderance optical properties and advantages of QDs. In addition, the principles of fluorescence quenching concerning ion complexing reaction, electron transfer, fluorescence resonance energy transfer and others, fluorescence enhancement and methods combined with rolling circle amplification of DNA, redshift of emission wavelength, fluorescence ratio and others were presented. Finally, the paper summarized the applications and took prospect to provide the basis for the detect