nanofibrous membrane a - 元智大学生物科技与工程研究所.pdf

13th Biochemical Engineering Conference Yuan Ze University PII-1 Immobilization of cellulase onto the electrospun poly(acrylonitrile-blending-maleic acid anhydride) nanofibrous membrane Man-Hsin Wang(王曼馨), Sheng-Feng Li( 李昇峰), Chun-Chong Fu(傅俊中 ), Wen-Teng Wu(吳文騰 )* Department of chemical engineering, National Cheng-Kung University, Tainan 70101, Taiwan *Fax: +886-6-2344496 E-mail: wtwu@.tw Abstract Electrospinning is the novel process to produce fibrous membranes with nanoscale diameters and properties of high specific surface area as well as porous structure, so electrospun nanofibrous membrane are excellent support for filtration, drug delivery, tissue engineering scaffold and enzyme immobilization. In this study, nanofibrous membrane, made by poly (acrylonitrile-blending- maleic acid anhydride) (PAN-b-MA), was containing with anhydride groups for immobilizing cellulase from Aspergillus niger . The average diameter of PAN-b-MA nanofiber was 200 nm observed by FESEM and covalent bond formation between enzyme molecule and nanofibrous membrane was confirmed by ATR detection. The biocatalytic efficiency of immobilized cellulase was examined for carboxymethyl cellulose (CMC) hydrolysis for finding the optimal immobilization conditions in appropriate enzyme concentration 10 wt%, pH value of 4.6 and 90 min at 60℃. When the blending ratio of PAN and maleic anhydride ratio was given as 1:1 (w/w), the immobilized cellulase hydrolyzed 2 % (w/v) CMC solution into 7.5 mg/ml glucose in 30 minutes. PAN-b-MA nanofibrous membrane was prepared by electrospinning and successfully used as the support for cellulase immobil