-岩溪晚芦 椪柑果皮与果肉差减cdna文库的构建及初步分析.pdf

2009, 36 ( 7): 967- 974 ActaHorticu lturae Sin ica -. cDNA * 叶庆亮, 江 东 , 彭爱红 ( , 400712) : -. ( Citrus reticulata Blanco) , ( tester) ( driver) cDNA : cDNA 100~ 500 bp 411, NCBI , 377 ES , 126, () gag ES 63 , 491 2% 5 62 Blast, 34 , : ; ( SSH ); cDNA ; ; ; : S 666; Q 785 : A : 0513-353X ( 2009) 07-0967-08 Construction of a Subtractive cDNA L ibrary Between FruitEpicarp andPulp of PonkanM andarin and Primary Analyses of t e Sequences * YE Q ing-liang, JIANG Dong , and PENG A -i hong (C itrus Research Institute, ChineseA cademy of A gricultural Sciences, Chongqing 400712, Ch ina) Abstract: A cDNA library was successfully constructed via suppression subtractive hybridization between fruit epicarp ( expermi ental group, tester) and pulp ( control group, the driver) of Ponkan (Citrus reticu la ta Blanco). Results showed that most of the clones had inserting fragmentswhich length ranged from 100 bp to 500 bp. Four hundred and eleven ES swere successfully sequenced and searched againstNCBIGenBank, the results indicated that 377 ES s had significant smi ilaritiesw ith known genes. A ll the ES s were divided into 126 genes according to the gene annotation. hese ES s were mainly involved in pigment synthesis, energy metabolism, prmi ary metabolism, secondary metabolism, defense against stresses, signal transduction, tran- scription, flavor form ation, oil synthesis, peel softening, antioxidantmetabolism etc. here were 63 uni-ES s which accounted for 4912% of the total ES s and another 34 ES s had no matching homologous sequences found. Key words: citrus; suppression subtractive hybridization ( SSH ); cDNA library; expressed sequence tag; clone; differentially expressed genes -. ( Citrus reticulata B lanco)