一种分析土壤中酚酸类物质含量的新方法 - 海藻活性物质国家重点实验室.pdf

2013,46(21):4612-4619 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.21.025 1 2 ( /271018 266400 3 201206) μ· A New Method for Analysis of Phenolic Acids in the Soil Soil from Replanted Apple Orchards was Investigated 1 1 2 3 1 1 YIN Cheng-miao , WANG Gong-shuai , LI Yuan-yuan , CHE Jin-shui , SHEN Xiang , CHEN Xue-sen , 1 1 MAO Zhi-quan , WU Shu-jing 1 ( College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology Taian 271018, Shandong; 2 Qingdao Bright Moon Bluesea Bio-Tech Co, LTD, Qingdao 266400, Shandong; 3 Thermo Fisher Scientific, Shanghai 201206) Abstract: ObjectiveTo establish a new protocol based on the ASE-HPLC method, which could determine the content of phenolic acids in orchard soil rapidly and efficiently.MethodPhlorizin was set as an sample for analysis, which is one of the characteristic phenolic acids in apple orchard soil. Accelerated solvent extraction (ASE) combined with high performance liquid chromatography (HPLC) was used to assess it. Specifically, the parameters of extraction solvent, extraction temperature, pressure and number of cycles were optimized, aiming at exploring the optimal conditions of ASE extraction for phenolic acid