非化感水稻响应低氮培养及共培稗草的上调表达基因分析 方长旬 .doc

非化感水稻低氮及稗草的表达基因分析(福建农林大学生命科学学院农业生态研究所 福州 350002) 摘 要 营养匮乏和伴生杂草是水稻种植过程中的常见影响因素。本研究应用抑制消减杂交（SSH）技术，分别研究低氮、稗草条件下，非化感水稻品种Lemont的上调表达基因。结果显示，低氮条件下，Lemont水稻中参与生长调控的生长素响应蛋白，参与抗逆防御的NBS-LRR蛋白、过氧化氢酶、金属硫因蛋白，以及参与蛋白质代谢的相关基因上调表达。稗草共培下，NADH脱氢酶、细胞色素氧化酶、氧类固醇结合蛋白等与植物生长相关的基因，与抗逆防御相关的几丁质酶和糖基水解酶基因，以及与信号转导相关的锌指蛋白及组氨酸激酶基因增强表达。研究结果，非化感水稻Lemont能够调节抗逆以及生长调节相关基因的表达，从而响应不同的。 水稻 低氮 稗草 基因表达accompanying barnyardgrass FANG Chang-Xun, Xu Tie-Cheng, Huang Li-Kun, Wang Qing-Shui, He Hai-Bin, Lin Wen-Xiong** (Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China) Abstract Nutrient deficiency and accompanying weed are general influence factors on rice growth. In this research, suppression subtractive hybridization (SSH) was used to investigate the up-regulate genes in non allelopathic rice accession Lemont that respectively in response to low nitrogen condition and accompanying barnyardgrass. The results showed that genes encode auxin-responsive protein which involved in plant growth and cell cycle regulation, NBS-LRR-like protein, catalase, metallothionein-like protein that involved in stress resistance, and protein metabolism relevant genes were up-regulated. When the rice was accompanied with barnyardgrass, genes encode NADH dehydrogenase, oxysterol-binding protein and cytochrome oxidase that relative to plant growth and cell cycle regulation, RING finger family protein and two-component sensor histidine kinase relative to signal transduction, chitinase and glycosyl hydrolases relative to rice resistance were increased expression. The findings suggest that rice survive in low nitrogen supply or barnyardgrass competition by increasing expression level of varies genes that correlate to enhancement of resistance and plant growth regulation. Key words Rice, Low nitrogen condition, Barnyardgrass accompanied, Gene expression 水稻(Oryza sativa L.)作为世界上最重要的粮食作物之一，是全球一半以上人口的主要食物来源之一。我国水稻的播种面积和总产量均居粮食作物首位，据统计，在近半个世纪中，全国水稻年播种面积占粮食种植总面积的27%，但