加杨树叶中3种黄酮类成分的含量测定-中国现代应用药学.PDF

加杨树叶中 3 种黄酮类成分的含量测定  * 刘影，梅晰凡(辽宁医学院，辽宁 锦州 121001) 摘要：目的 建立同时测定加杨树叶中的槲皮素、高良姜素、白杨素含量的分析方法。方法 采用高效液相色谱法，色 谱柱Agilent TC C18 柱(4.6 mm×250 mm ，5 μm) ，流动相：甲醇-0.1% 甲酸，梯度洗脱：0~5 min ，甲醇25%→55% ，5~25 min ， 1 甲醇55%→65% ，25~35 min ，甲醇65%→75% ；流速：1.0 mL·min ；检测波长：264 nm ；柱温：30 ℃。结果 加杨树 叶中槲皮素、高良姜素、白杨素分离效果良好，线性范围分别为3.7~120 ，2.5~80 和 3.125~100 μg·mL1 ；平均加样回收 率分别为99.0% ，100.1%，101.4%。结论 该方法准确可靠、结果稳定、重复性好，可为评价加杨树叶的质量提供依据。 关键词：加杨树叶；槲皮素；高良姜素；白杨素；高效液相色谱法 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2014)07-0857-04 DOI: 10.13748/ki.issn1007-7693.2014.07.022 Determination of Three Flavonoids in Populus Canadensis Moench. Leaves * LIU Ying, MEI Xifan (Liaoning Medical University, Jinzhou 121001, China) ABSTRACT: OBJECTIVE To establish an analytical method for simultaneous determination of quercetin, galangin and chrysin in Populus canadensis Moench. leaves. METHODS The determination was performed on Agilent TC C 18 column (4.6 mm ×250 mm, 5 μm) with the mobile phase consists of methanol-0.1% formic acid at a flow rate of 1.0 mL·min–1 . The following gradient elution was used: the content of methanol was 25% to 55% from 0 to 5 min, 55% to 65% from 5 to 25 min and 65% to 75% from 25 to 35 min. The detection wavelength was set at 264 nm, and the column temperature was 30 ℃. RESULTS This method had a good separation performance for quercetin, galangin and chrysin in poplar leaves, and the linear range were 3.7120, 2.580 and 3.125100 μg·mL1, respectively; the average recovery rates were 99%, 100.1% and 101.4%, respectively. C