茄子品种抗黄萎病性鉴定方法研究_叶鹏盛.pdfVIP

西　南　农　业　学　报 2006 19 6 　　　　　　　　　　　　　　 1066 Sou thw est Ch ina Journa of A gricu tu ra Sciences V o .19　　N o.6 :100 1 - 4829(2006)06 - 1066 - 05 叶鹏盛, 曾华兰,李琼芳,何　炼,韦树谷 (, 　64 1400 ) 　:本文通过研究茄子黄萎病抗性鉴定方法, 明确了茄子黄萎病抗性鉴定的接菌方法、接菌量、接菌时期以及抗性型划分 7 - 1 准。结果表明, 将黄萎病菌培养制成 2×10 mL 的孢子悬浮液, 在茄苗 3 ～4片真叶移栽时伤根接菌, 按每株 10 m L 的量施用孢 子悬浮液接种黄萎病菌, 接菌后60 d, 按 5 级法分级调查各品种的黄萎病发生情况, 根据病情指数可以确定品种的抗性型。利用 该方法对 31 个品种进行鉴定, 不同抗性反应型品种的病情指数差异显著, 鉴定结果与多年生产上的抗病性表现 一致, 表明该方法 鉴定结果准确可靠, 能客观反映茄子品种的抗病性能。 :茄子;黄萎病;抗病性;鉴定方法 :S 322. 1;S64 1. 1　　　:A Study on evaluationm ethod of eggplant variety resistance to Vertic illium w ilt YE P eng-sheng, Z ENG Hu -a an, LI Q iong-fang, H E L ian, W E I Shu-gu (Indu stria C rops Inst itu te, S ichuan A cadem y of A gricu tu ra S ciences, S ichuan Jianyang 64 1400, C hina) Abstract:T he eva uat ion m ethod of eggp an t variety res is tan ce to Vertici llium w ilt was stud ied. It had been estab ished by s tudy ing the su -it ab e inocu ating m ean, dosage, stage and resistance type. The d isease w ou d fu y occur 60 day s after inocu ation at 3 - 4 true eaf stage w hen 7 - 1 the seed ings w ere transp an ted, w ith 10 mL of Verticillium dah liae spores suspension (2×10 m L )per p an t by w atering the inju red roots. T he p an t sym p tom s w ere c assified into 5 grades. Th e resistance type of each variety w as determ ined by d isease ind ex. R es is tan ce of 31 varieties w as eva u ated us ing th is m ethod, the resu t show ed th at the m ethod w as effective and ex act. Key words:eggp an;t Verticil lium w il;t d isease resis tan ce;eva u ation m e