醋酸对巴氏醋杆菌生长和代谢活性的影响.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.9 醋酸对巴氏醋杆菌生长和代谢活性的影响 郑宇，姜春悦，陈兴京，杨晟，王敏 （工业发酵微生物教育部重点实验室，天津科技大学生物工程学院，天津300457） 摘要：醋酸对微生物具有较高的毒性，醋酸菌具有特殊的机制能够耐受较高浓度的醋酸，常用于醋酸的发酵生产。本论文以巴 氏醋杆菌AC2005 为研究对象，研究了醋酸对菌体生长、葡萄糖代谢和胞内ATP 浓度的影响。与不含醋酸条件下相比，培养基中加 入1% （V/V）的醋酸，菌体的生物量增加了92%，单位菌体葡萄糖消耗速率增加了13%，细胞代谢活性增加了120%，代谢产物中丙 酮酸浓度增加了约46%，柠檬酸和琥珀酸的浓度分别降低了80%和46%，胞内ATP 浓度增加了 171%。进一步利用液相色谱串联质 谱法（LC-MS/MS）的方法分析了蛋白表达情况，发现加入醋酸后细胞内与三羧酸循环相关的蛋白明显上调。结果表明，醋酸菌可以 通过提高菌体代谢活性，增加胞内ATP 浓度，从而耐受较高浓度的醋酸。 关键词：巴氏醋杆菌；醋酸耐受性；三羧酸循环；代谢活性 文章篇号：1673-9078(2014)9-149-153 DOI: 10.13982/j.mfst.1673-9078.2014.09.025 Effects of Acetic Acid on the Growth and Metabolic Activity of Acetobacter p asteurianus ZHENG Yu, JIANG Chun-yue, CHEN Xing-jing, YANG Sheng, WANG Min (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China) Abstract: Acetic acid is toxic to micro organisms; however, acetic acid bacteria can tolerate high concentrations of acetic acid via a specific mechanism and, therefore, are ideal for use in acetic acid fermentation. In this study, Acetobacter pasteurianus AC2005 was used to evaluate the effect of acetic acid on bacterial growth, glucose metabolism, and intracellular ATP concentration. The results showed that addition of 1% (v/v) acetic acid resulted in an increase in biomass by 92%, glucose consumption per unit mass by 13%, cell metabolic activity by 120%, intracellular ATP concentration by 171%, and pyruvic acid concentration in metabolites by approximately 46%, whereas a decrease in concentration of citric acid and succinic