热休克蛋白27对脊神经根撕脱伤后脊髓运动神经 - 第三军医大学学报.DOC

热休克蛋白27对大鼠臂丛神经根撕脱伤后脊髓运动神经元细胞凋亡的影响 刘奕蓉1 赵国志2 赵毓芳1 吴建中3 支晔3 （1华北煤炭医学院 2 唐山市第二医院 3 汕头大学医学院解剖学教研室） 摘要 目的：观察热休克蛋白27（heat shock protein 27，Hsp27）对大鼠臂丛神经根撕脱后脊髓前角运动神经元细胞凋亡的影响。方法：健康雄性Wistar大鼠60只，随机分为实验组和对照组。实验组动物分别以45℃热休克预处理15min，再置于42℃维持20min，置室温恢复24h后，在手术显微镜下进行脊髓C5~T1节段神经根撕脱术，分别于术后12h、1d、3d、5d、7d处死动物。对照组仅施行臂丛神经根撕脱术，并分别于术后12h、1d、3d、5d、7d处死动物。各组分别进行Hsp27免疫组织化学染色和原位末端标记法（TUNEL）检测脊髓运动神经元细胞凋亡情况，并结合自动图象分析系统对结果进行半定量分析。结果：两组Hsp27表达的高峰均在热休克后1d，且实验组强于对照组；实验组脊髓运动神经元细胞凋亡在1d达到高峰，而对照组凋亡细胞的高峰出现在3d，且凋亡细胞数明显高于实验组。结论：臂丛神经根撕脱伤后热休克蛋白27可抑制脊髓运动神经元凋亡。 关键词 热休克蛋白27；脊髓；运动神经元；细胞凋亡 中图分类号 R 322.81 文献标识码： A Effect of heat shock protein 27 on apoptosis of spinal cord anterior horn after brachial plexus avulsion in rats LIU Yirong1, ZHAO Guozhi2 ZHAO Yufang1 WU Jianzhong3, ZHI Ye3 Abstract Objective：To observe the effect of heat shock protein 27 on apoptosis of spinal cord anterior horn after brachial plexus roots avulsion. Methods：Sixty adult Wistar male rats were divided into control and experiment group at random. The experiment group subjected to heat shock under 45℃ for 15min，and maintained under 42℃ for 20min subsequently. After recovering 作者简介 刘奕蓉，女，包头市人，硕士，副教授， 硕士生导师，主要从事中枢神经损伤研究 E-mail: liuyirongts@126.com telephone:24h under the room temperature, the nerves of brachial plexus were avulsion with microhemostatic forcep. In a span from 12h to 7d, these animals were killed at different time. But the control group only received the surgery of the nerveroots of brachial plexus avulsion. Apoptosis was detected by TDT-mediated dUTP nick end labeling （TUNEL），the immunohistichemical method was used to detect leveling the expression of Hsp27. Results: 1. The hsp27 begin to express at 12h and significantly increased the level with a peak at 1d in two group, But the control group contained a lower level in the expression of hsp27 than the experiment group. 2. TUNEL results showed the peak of apoptosis at 1d in experiment groups，and in control groups ，the p