胃肠运动机制.ppt

* * * * * * * * * * * * * * * * * 配伍后成分药代动力学 胃肠安丸组方中药味配伍药动学 为了避免血浆中的内源性杂质对所检测血样中和厚朴酚及厚朴酚的干扰，实验中考察了不同血浆样品处理方法处理。血浆蛋白沉淀以甲醇、乙腈沉淀除蛋白；液液萃取；固相萃取小柱处理四种方法进行处理，比较萃取回收率及基质干扰。 固相萃取小柱处理 配伍后成分药代动力学 胃肠安丸组方中药味配伍药动学 Figure 2. Chromatography of magnolol, honokiol and IS in rat plasma with LC-MS/MS. A: the blank plasma; B: the blank plasma spiked with the standards; C: the plasma sample after 1 h oral administration of WCA. a: TIC chromatogram in negative ESI mode; b: MRM spectrums of honokiol; c: MRM spectrums of magnolol; d: MRM spectrums of IS. 配伍后成分药代动力学 胃肠安丸组方中药味配伍药动学 No. Components Regression equation R2 Linear range （ng/mL） LOD （μg/mL） LOQ （μg/mL） 1 Honokiol y = 8.551x – 0.0437 0.997 5.25-1344.00 2 Magnolol y = 13.738x - 0.07053 0.998 18.064-5429.69 Table 1 Statistics results of linear regression equation analysis in the determination of the markers. 配伍后成分药代动力学 胃肠安丸组方中药味配伍药动学 Table 2 Precision and accuracy of the assay for honokiol and magnolol in rat plasma. Components Spiked concentration （ng/mL） Inter-day Intra-day 测定值 RE % RSD /% 测定值 RE % RSD /% Honokiol 10.50 9.80 6.14 -6.68 10.31 7.92 1.79 84.00 85.74 2.90 2.08 83.77 3.14 0.27 672.00 675.72 1.96 0.55 669.94 3.70 0.31 Magnolol 32.47 31.76 5.48 -2.18 32.74 5.15 -0.84 342.63 351.64 4.26 2.63 358.00 2.62 -4.48 2785.00 2604.11 7.07 -6.50 2711.27 6.94 2.65 配伍后成分药代动力学 胃肠安丸组方中药味配伍药动学 Table 3 Stability of honokiol and magnolol in rat plasma. Components Concentration added (μg/mL) Accuracy (%, mean±S.D.) 24 h at room temperature Freeze-thaw cycles 3 times 30 days storage at -20 ℃ Honokiol 10.50 97.84±6.48 99.89±2.73 92.01±8.78 84.00 97.93±3.49 98.45±4.03 104.36±9.46 672.00 99.89±5.42 100.76±4.68 98.18±6.99 Magnolol 32.47 101.03±5.41 100.09±3.12 99.06±4.74 342.63 99.85±3.64 98.80±1.20 99.85±3.64 2785.00 100.52±4.42 98.00±1.08 100.52±4.42 配伍后成分药代动力学 胃肠安丸组方中药味配伍药动学 Table 4 Recovery of honokiol and magnolol in rat plasma. Components Spiked concentration （ng/mL） Recovery (%) RSD (%) Honokiol 10.50