大鼠胫神经损伤腓肠肌组织中Myostatin的表达变化.pdfVIP

UU墨生 22鲞 _l期 Med J of Communications．20P08．Vo1．22．No．．1 [文章编号]1 0(I6—2440(2oo8)01—00o1_03 ·论著与基础研究· 大鼠胫神经损伤腓肠肌组织中Myostatin的表达变化 黄 丽 王 洁 孙华林 梅晓云 (南通大学江苏省神经再生重点实验室，江苏226001；南京中医药大学，江苏210029) 摘 要 目的：分析myostatin(MSTN)在大鼠胫神经夹伤后腓肠肌失神经萎缩与神经再支配过程中的表达变 化，探讨myostatin在失神经肌萎缩过程中的可能作用。方法：建立大鼠胫神经夹伤模型：采用RT—PCR法检测失 神经与神经再支配不同时间段腓肠肌中myostatin mRNA含量。结果：失神经支配早期，腓肠肌myostatin mRNA水 平迅速上升，在第2周达到高峰，随后表达又逐渐下降，至第4周与正常组相比差异无统计学意义( 0．05)。结论： 成功建立大鼠胫神经夹伤模型，腓肠肌失神经肌萎缩过程中，myostatin基因表达有明显变化，提示myostatin在失 神经肌萎缩过程中扮演重要角色。 关键词 iyostatin；失神经支配；肌萎缩；逆转录一聚合酶链反应 中图分类号 R392-一33 文献标识码 A Expression of myostatin in rat gastrocnemius during denervation of tibial HUANG Li，WANGJie，SUN Hualin，MEI Xiaoyun(Jiangsu Key Laboratory of Neumregeneration,，Nantong University,Jiangsu 226001； Nanjing University of Traditional Chinese Medicine,Jiangsu 210029) Abstract Objective：To investigate myostatin(MSTN)expression pattern and its potential role in atrophic gastrocnemius during denervation and reinnervation．Methods：The model of rat tibial nerve crush was successfully established and myostatin mRNA levels in atrophic gastmcnemius were detected by RT-PCR．Results：The results showed that in the early stage of denervation，the level of myostatin mRNA in the denervated gastrocnemius muscle increased instantly，reaching a peak during the second week after being crushed，and then returned to its normal level gradually until the 4th week after the tibial nerve was crushed(P0．05)．Conclusions： The model of rat tibial nerve crush was successfully established．There is a significant change of the myostatin gene in the denervated gastmcnemius muscle after being crushed．which indicats that myostatin may play a significant role in muscle atrophy after denervation． ， Key words My