大鼠海马的取材.pdf

丁 香 园  zhuojianfan 制作 大鼠海马的取材 step 1:This is a dorsal view of a mouse’s skull, in order to access the brain you can cut along the coronal suture and sagittal suture then pull off both sides of parietal bone and interparietal bone. 1  丁 香 园  zhuojianfan 制作 step 2: Now the skull is open and you can see the brain clearly, in order to get the hippocampus you’d better keep the brain in the cranial cavity so the brain will not move when you continue with the following steps. 2  丁 香 园  zhuojianfan 制作 step 3: In order to expose the hippocampus you need to remove the cerebral cortex covering it. The first incision is at the end of the hemisphere; the incision should be about 0.7mm deep for most adult mouse that you might not hurt the hippocampus while to expose it. The 2nd incision is about 1.5-2mm in front of the first one, this incision you need cut into the lateral ventricle, both of the incisions go to the ventral of the brain and meet there. Now this piece of cortex is free, pull it up, you will see the hippocampus just like in this picture, also you can see the CSF in the opened ventricle. 3  丁 香 园  zhuojianfan 制作 step 4: Keep working on the other side of the brain pull up both sides of the cortex that covering the hippocampus along the ventricle. Now you can see the dorsal part of the hippocampus. S