甘油对粒毛盘菌dp5 胞外多酚积累的影响.pdf

微生物学通报 Jan. 20, 2016, 43(1): 140−146 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150254 研究报告 甘油对粒毛盘菌DP5 胞外多酚积累的影响 * 杨柳 曹焕英 孙丹宇 邓杰勇 吴俊贤 叶明 (合肥工业大学 生物与食品工程学院 安徽 合肥 230009) 摘 要：【目的】探明以甘油为碳源促进粒毛盘菌DP5 积累多酚的可能原因。【方法】对碳源种 类、甘油浓度、曲酸、抑制剂和前体等对多酚产量和生物量的影响进行分析。【结果】以甘油为 碳源，能显著提高粒毛盘菌胞外多酚产量。甘油浓度为20 g/L 时，胞外多酚产量最高，达到 0.664 g GAE/L ，并在发酵液中检测到曲酸，其含量为0.25 g/L。向以蔗糖为碳源的发酵液添加曲 酸，胞外多酚含量从0.209 g GAE/L 提高至0.376 g GAE/L。以甘油为碳源的发酵液中，酚氧化 酶活性较低。粒毛盘菌 DP5 通过莽草酸途径和聚酮途径合成多酚，甘油有利于莽草酸途径和聚 酮途径前体物质的合成。【结论】粒毛盘菌以甘油为碳源合成出曲酸，曲酸抑制多酚向黑色素的 转化；甘油促进多酚前体的合成，从而提高了粒毛盘菌胞外多酚的积累量。 关键词：粒毛盘菌，胞外多酚，曲酸，莽草酸途径，聚酮途径 Effect of glycerol on extracellular polyphenols accumulation in Lachnum DP5 * YANG Liu CAO Huan-Ying SUN Dan-Yu DENG Jie-Yong WU Jun-Xian YE Ming (College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China) Abstract: [Objective] The possible relationships between glycerol and the extracellular polyphenols (EP) production from Lachnum DP5 were explored initially. [Methods] The influences of various carbon sources, glycerol concentration, kojic acid, inhibitors and precursors on EP production and biomass were investigated. [Results] The EP accumulation was obviously increased by the addition of glycerol. The maximum EP content reached 0.664 g GAE/L when using 20 g/L glycerol as the carbon source. Meanwhile, kojic acid was produced, and its yield was 0.25 g/L. The EP yield increased from 0.209 g GAE/L to 0.376 g