回转模拟失重对心肌成纤维细胞ⅰ型胶原代谢的影响3 - current .pdf

动物学报 　50 (2) :252 - 257 , 2004 A cta Zoologica S inica 　　　　　 　 回转模拟失重对心肌成纤维细胞 Ⅰ型胶原代谢的 影响 杨　芬　李莹辉 　戴钟铨　聂捷琳　熊江辉 航天医学工程研究所 , 北京 100094 摘　要 　为探讨模拟失重对心肌成纤维细胞 Ⅰ型胶原代谢的影响 , 本研究采用回转器模拟失重效应 , 通过免疫 细胞化学和反转录聚合酶链式反应 ( RTPCR) 研究了回转模拟失重对原代培养的新生大鼠心肌成纤维细胞 Ⅰ型 胶原蛋白及 mRNA 表达 , 以及胶原降解抑制物 ———金属蛋白酶组织抑制因子 ( Tissue inhibitor of metallopro teinase , TIMP) mRNA 表达的影响。免疫细胞化学染色显示回转组 Ⅰ型胶原蛋白沉积增加 ; RTPCR 分析显示 α ( α ) Ⅰ型胶原 1 链 Type Ⅰcollagen 1 chain , Col ⅠA1 mRNA 表达没有明显变化 , TIMP1 、TIMP2 及 TIMP3 的 mRNA 表达均增强。提示在回转模拟失重条件下 , 心肌成纤维细胞 Ⅰ型胶原蛋白沉积增加 , 作为胶原降解抑制 物的 TIMP 可能是造成胶原沉积的原因 [动物学报 50 (2) : 252 - 257 , 2004 ] 。 关键词 　大鼠 　回转器 　心肌成纤维细胞 　胶原 Effects of clinorotationinduced weightlessness on the metabolism of type Ⅰcollagen in cultured cardiac f ibroblasts YAN G Fen , L I YingHui , DAI ZhongQuan , N IE JieLin , XION G JiangHui Institute of Space MedicoEngineering , Beijin g 　100094 , China Abstract 　Researches on the effects of weightlessness/ simulated weightlessness on the heart have focused on the contrac tile function , and little is known about changes in myocardial interstitial matrix. We investigated whether gene expression and protein deposition of type Ⅰcollagen in cardiac fibroblasts were affected by clinorotationinduced weightlessness. Neonatal rat cardiac fibroblasts were obtained by stepwise trypsin dissociation and cultured in Dulbecco’s minimal essential medium (DMEM) supplemented with 1