bt杀虫晶体蛋白的肠溶性微胶囊研究.doc

正交试验法研究Bt杀虫晶体蛋白的肠溶性微胶囊配方 阮传清1，张明政2，刘芸1，朱琳珊2，刘波1 （1福建省农业科学院农业生物资源研究所，福州　350003；2 福建农林大学，福州 350002） 摘要：采用双乳液法研制苏云金芽孢杆菌(Bacillus thuringensis，简称Bt)晶体蛋白的肠溶性微胶囊，并应用L16(45)正交试验设计研究了相关因素对微胶囊包封率的影响。结果表明，5个因素对包封率均有显著影响，影响大小依次为芯材乳化程度Bt晶体蛋白浓度(芯材)HPMCP浓度(壁材)蓖麻油浓度(助剂)甲基纤维素浓度(亲水胶体)。以500 mL 分散系为条件，筛选出的肠溶性微胶囊较佳工艺为：芯材乳化转速6000 r/min， 20% Bt晶体蛋白溶液1mL，6% HPMCP溶液 50mL，分散系中含0.4% 蓖麻油和2.2-2.5%甲基纤维素钠。按此种较佳组合制备微胶囊，对Bt晶体蛋白的包封率在85%左右，微囊直径约为20.7 μm，在pH 8.5 的碱性缓冲液中可在5分钟内崩解，释放出Bt晶体蛋白。 关键词：苏云金芽孢杆菌(Bacillus thuringensis)，肠溶性微胶囊，Bt伴孢晶体蛋白 Studies on the preparation technology of enteric microcapsule of insecticidal crystal proteins from Bacillus thuringensis by orthogonal matrix method RUAN Chuan-qing1, ZHANG Ming-zheng2, LIU Yun1, ZHU Lin-shan2, LIU Bo1 (1 Institute of Agro-biological Resources, Fujian Academy of Agriculture Sciences, Fuzhou 350003; 2 Fujian Agriculture and Forestry University, Fuzhou 350002) Abstract: Enteric microcapsule with insecticidal crystal proteins (ICP) of Bacillus thuringensis as core material and hydroxypropyl methylcellulose phthalate (HPMCP) as shell material was prepared by double emulsion method. Experiments in orthogonal matrix design [L16(45)] showed that the encapsulation of ICP was influenced by factors such as emulsification rotation speed, concentration of core and shell materials, emulsification agent and hydrocolloids, ranged in a pattern of decreasing importance. In 500 mL aqueous medium for formation of the microcapsule, the optimal condition was 6000 r/min of emulsion rotation, 1 mL solution of 20% IPC, 6 mg of HPMCP dissolved in 50 mL mixture of ethanol and acetone (1:1 v:v), 2 mL castor oil and 2.2-2.5%carboxymethyl cellulose (CMC-Na) in aqueous medium, where about 85% ICP was encapsulated. The microcapsule was sphericity figure in diameter of mean 20.7 μm and releaved all protein within 5 min at Ph 8.5 medium. Keywords: Bacillus thuringensis, enteric microcapsule, Bt crystal proteins 苏云金芽孢杆菌(Bacillus thuringiensis，简称Bt)因具有绿色、环保等优点而倍受青睐，成为了目前研究最深，应用