药材与资源.PDF

中草药 Chinese Traditional and Herbal Drugs 第 46 卷 第 6 期 2015 年 3 月 • 881 • • 药材与资源 • 罗汉果三倍体雌株与二倍体雄株遗传背景的ISSR 分析 1, 2 1* 韦荣昌 ，白隆华 1. 广西药用植物园，广西 南宁 530023 2. 中国医学科学院 北京协和医学院药用植物研究所，北京 100193 摘 要：目的 探索罗汉果 Siraitia grosvenorii 三倍体雌株与二倍体雄株的遗传背景，为无籽罗汉果优良品种的选育提供分 子生物学依据。方法 利用 ISSR 分子标记对 28 份罗汉果种质进行遗传背景分析，用 AFLP-SURV 计算材料间遗传距离；用 TSYS-pc 和 GenAIEx 分别进行聚类和主坐标分析。结果 从 100 条 ISSR 引物中筛选出 13 条用于扩增，共获得 131 条清晰 可辨条带，其中多态性条带 99 条，多态性位点百分率为 75.57% 。聚类和主坐标分析表明罗汉果三倍体雌株和二倍体雄株的 遗传背景存在一定的丰富性，但大多遗传相似性系数较大，遗传距离较近。结论 罗汉果三倍体雌株和二倍体雄株遗传背景 的复杂性较低，应该尽快采取相应措施，进行种质创新，丰富无籽罗汉果亲本的遗传背景。 关键词：罗汉果；二倍体；三倍体；遗传背景；ISSR 中图分类号：R282.12 文献标志码：A 文章编号：0253 - 2670(2015)06 - 0881 - 06 DOI: 10.7501/j.issn.0253-2670.2015.06.019 ISSR analysis of genetic background of triploid female and diploid male strains of Siraitia grosvenorii WEI Rong-Chang1, 2, BAI Long-hua1 1. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100193, China Abstract: Objective To evaluate the genetic background of triploid female and diploid male strains of Siraitia grosvenorii and provide the biological reference for good varieties breeding of seedless S. grosvenorii . Methods Inter simple sequence repeats (ISSR) marker was developed to analyze the genetic background among 28 samples of S. grosvenorii, and cluster analysis and double principal coordinate analysis were revealed by the NTSYS-pc software and GenAIEx software, respectively. Results Out of 100 ISSR primers selected, 13 primers were used for amplification and a total of 131 unambiguous bands were obtained, among which 99 (PPB = 75.57%) were polymor