乳酸羟基乙酸共聚物微泡声学造影剂的制备与评价 - 第三军医大学学报.docVIP

乳酸羟基乙酸共聚物微泡声学造影剂的制备与评估实验研究 李奇林，全学模 （400014 重庆,重庆医科大学附属儿童医院超声科） [摘要] 目的 制备乳酸羟基乙酸共聚物(poly lactic-co-glycolic acid ,PLGA)微泡声学造影剂，考察其理化及声学特性。方法 复乳法制备不同PLA/PGA的微泡，考察其理化特性；体内外显影评价不同剂量、不同机械指数条件下微泡造影剂的声学信号强度变化规律。结果 PLGA微泡成品呈白色粉末状，以生理盐水或PBS稀释溶解后为乳白色混悬液,pH为6.5士0.3；光镜显示微泡大小均匀，分散程度好；微泡平均粒径1.2μm； Coulter计数显示0.01 g多聚体微泡冻干粉溶于10 ml蒸馏水中的浓度为（2～echanical index, MI）呈直线相关关系；兔心脏造影检查可观察到PLGA微泡造影剂能顺利通过肺循环实现左心显影，当MI为0.2时，左室内声学密度值较其他3组（MI为0.6、1.0、1.2）均有非常显著性差异。结论 PLGA微泡造影剂具有较高的可控性和稳定性，微泡共振频率较高，可以顺利通过肺循环实现左心室显影。 [关键词] 声学造影剂，乳酸羟基乙酸共聚物，理化性质 [中图法分类号] R445.1 [文献标识码] A Experimental Study on poly lactic-co-glycolic acid microbubble ultrasonic contrast agent preparation Li Qilin，Quan Xuemo (Department of Ultrasonography，Children’s Hospital of Chongqing Medical University,Chongqing 400014,China) [Abstract] Objectives To Prepare PLGA microbubbles and evaluate its physicochemical and acoustic properties.Methods We prepared PLGA microbubbles by double emulsion method .We examined its physicochemical properties and evaluated its acoustic properties in vitro under different doses with ultrasound energy at a variety of MI values. Result PLGA microbubbles were white powder and ivory suspension dissolved in saline or PBS. Under microscope, microbubbles had uniform sizes and good dispersion with pH value of suspension 6.5士0.3..The microbubble suspension contained 2.0×109～4.0×109 /ml microbubbles, with mean diameter of 1.2μm. There were no significantly differences on concentration, diameter and pH value compared the group of 4℃ with the group at common temperature. The vitro study showed that acoustic intensity increased with PLGA microbubbles concerntration and changed with MI linearly. We observed that PLGA microbubbles had passed through pulmonary circulation and achieved left ventricular imaging in vivo. There were significantly differences on acoustic intensity of left ventricular compared the MI 0.2 group with the other three groups(MI 0.6,1.0,1.2). Conclusion PLGA microbubbles