日本血吸虫尾蚴与直链有机化合物的亲和性分析及富集装置初步设计.pdfVIP

Study of the affinity between the schistosome cercariae larvae and organic compounds and primary design of the enrichment device for the cercariae larvae Abstract Schistosome cercariae larvae is the unique stage infecting human beings and mammals. In order to forecast the epidemic situation in real time, a series of study were carried out to detect the schistosoma cercariae. Firstly, the affinity of schistosoma cercariae larvae was studied using a series of compounds in this experiment. Each kind of compound was dissolved to be 1mmol/L solution. Then, the solution was spread to form the membranes on a 75mm×25mm leave board. The affinity of schistosoma cercariae with each compound was judged by the capture ratio of cercariae. The results indicated that the n-caprylic acid and linoleic acid had the better affinity to schistosoma cercariae. The equal absolutely capture ratio of cercarciae could respectively achieve to 52.2% and 59.4%. Secondly, the polypropylene (PP), the polyethylene (PE), the Ethylene/vinyl acetate (EVA) and the polyurethanes (PVP) thin films were used to stick the schistosome cercariae. The schistosoma cercariae which stick by the polymers films were observed by the color TV microscope. The results shown that the polypropylene (PP) had the better affinity to the schistosome cercariae, and the equal absolutely capture ratio of cercarciae might achieve to 29.7%. Then the better affinity molecules were selected out to assemble on the high polymer thin films. The polypropylene (PP) that assembled with the n-caprylic acid was observed got high capture ratio of cercarciae, and the equal absolutely capture ratio of cercarciae was reached up to 49.6%. According to the natural distribution of schistosome cercariae in the water, a set of enrichment device was designed