egfr和anxa 2在不同级别人脑胶质瘤中的表达 - 第三军医大学学报.doc

ANXA 2和EGFR在人脑胶质瘤中的表达及临床意义 杨斌 刘献志 李进军 辛艳超 翟瑛 450052 郑州，郑州大学第一附属医院神经外科 [摘要] 目的 研究EGFR和ANXA 2信号通路在不同级别人脑胶质瘤组织中的表达情况及临床意义。方法 采用免疫组化S-P法分别对38例低级别胶质瘤(Ⅰ、Ⅱ级)和38例高级别胶质瘤（Ⅲ、Ⅳ）以及10例正常脑组织组中的ANXA 2和EGFR的表达情况进行检测。并分析EGFR和ANXA 2表达情况及其相关性。结果 与正常脑组织相比EGFR和ANXA 2在人脑胶质瘤中的表达均显著增加。通过阳性区平均积分光密度定量分析,在正常组、低级别、高级别EGFR 和 ANXA 2数值分别为（106.49±5.69和103.52±3.75、124.96±3.39和125.62±2.37、149.66±4.63和148.23±5.29） ，各组之间EGFR和ANXA 2的测定值有显著差异（F=86.58和101.83，P0.05)。并且EGFR和ANXA 2表达也呈现显著正相关（r=0.521 ,P0.05)。结论 EGFR和ANXA 2在人脑胶质瘤中的表达均显著增加，且其表达具有的相关性。EGFR和ANXA 2信号通路可能在人脑胶质瘤的发生发展中起着一定的协同作用。 [关键词] 人脑胶质瘤；EGFR；ANXA 2；免疫组化 [中国法分类号] R394.2;R730.23;R739.41 Expression of ANXA 2 and EGFR in cerebral glioma and its clinical significance Yang bin,Liu Xianzhi,Li jinjun,Xin Yanchao,Zhai ying(Department of Neurosurgery,First Affiliated Hospital,Zhengzhou University,Zhengzhou,Henan Province,450052) 通讯作者：刘献志(郑州大学第一附属医院神经外科主任医师) 电话：0371 E-mail:lxz@zzu.edu.cn [Abstract]objective To study the expression of Epidermal Growth Factor Receptor(EGFR) and ANXA 2 signal pathway in human cerebral glioma and its clinical significance.Methods xpression of EGFR and ANXA 2 was detected in 45 glioma tissue samples ,33 advanced glioma tissue samples,and 20 normal brain tissue samples by S-P immunohistochemistry.Correlation between EGFR and ANXA 2 expression and related clinical data was analyzed .Results The expression level of EGFR and ANXA 2 was significantly higher in human cerebral glioma tissue samples than in normal cerebral tissue samples.Through the quantitative analysis of the average integral light density of positive district,the numerical value of EGFR and ANXA 2 in normal group,low level group,high level group respectively was 106.49±5.69 and 103.52±3.75、124.96±3.39 and 125.62±2.37、149.66±4.63 and 148.23±5.29. The values of EGFR and ANXA 2 expressed in human cerebral glioma had a significant difference at different levels(F = 86.58 and 101.83, P 0.05),and there was also a positive correlation between EGFR and ANXA