胰岛素样生长因子基因在鹅不同发育时期皮肤及 - 吉林农业大学学报.pdf

吉林农业大学学报 2011, 33( 1) : 60~ 63, 68 http : / / xuebao.j lau. edu. cn Journal of Jilin Agricultural University Email :j lndxb@ vip . sina. com 1 1 1 2 1 孙永峰, 史旭升 , 隋玉健, 刘少西 , 高 光 1吉林农业大学动物科学技术学院, 长春 130118; 2宁波出入境检验检疫局, 宁波315012 : 以吉林白鹅和籽鹅为试验对象, 采用RT- PCR 及免疫 织化学技术, 检测 28, 56 日龄鹅皮肤 织中 IGF 基因表达和定位情况结果表明: IGF mRNA 表达于上述两 个日龄的皮肤 织中IGF 特异性免 疫染色明显表达于28 日龄吉林白鹅和籽鹅胸部皮肤毛根鞘的扁平细胞和复层细胞, 腹部皮肤内根鞘外根鞘 及毛小皮在56 日龄2 鹅种胸部和腹部皮肤扁平细胞复层细胞皮质和髓质中也检测到IGF 蛋白免疫活 性在上述鹅种不同日龄不同 织细胞间IGF 蛋白免疫活性的表达强度明显不同 : 胰岛素样生长因子 ; 皮肤; 毛囊; 表达; 鹅 : Q786; S835 : A : 2011) 0 1006004 Study on the Expression of InsulinLike Growth Factor in Skin and Hair Follicle of Different Development Phases for Goose Tissues 1 1 1 2 1 SUN Yongfeng , SHI Xusheng , SUI Y ujia n , LIU Shaoxi , GAO Gua ng 1. College of Animal Science and Technology , JilinAgricultural Univ ersity , Changchun 130118, China ; 2. N ingbo EntryExit nsp ection and Quarantine Bureau of PRC, N ingbo 315012, China Abstract: In the e periment, with RT- PCR and immunohistochemistry, the e pression and location of IGF mRNA in skin and hair follicle from different development phases of geesewere detectedThe results showed that the mRNA and protein of IGF were present in the above two tissues of two daysThe spe cific immunostaining of IGF was mainly e pressed in flat cell and amblayer cell of root sheath of chest skin tissues of 28 days Jilin white geese and Zi geese, and was detected in the internal root sheath, outer rootsheath and hair cuticle of skin of abdomen; the immunostaining of IGF was also detected in the flat cell and amblayer cell corte and medulla of