凝胶渗透色谱-高效液相色谱法测定蔬菜中呋喃丹的含量.pdf

7 5 Vol. 7 No. 5 2016 5 Journal of Food Safety and Quality May , 2016 - 冯晓青, 汪 怡, 王 芹, 王 露, 宋 鑫, 杭学宇* (, 223001) 摘 要: 目的 -方法 -, C18 , , 285 nm, 320 nm, 结果 0.2~5.0 ng/mL , r 0.999, 0.01 mg/kg 3 0.5 1.0 2.0 mg/kg , 78.3%~97.6%, 3.26%~11.69%结论 , 关键词: ; ; ; Determination of carbofuran in vegetables by gel permeation chromatography-highperformance liquid chromatography * FENG Xiao-Qing, WANG Yi, WANG Qin, WANG Lu, SONG Xin, HANG Xue-Yu (Huai ’an Center for Disease Control and Prevention, Huai ’an 223001, China) ABSTRACT: Objective To establish a method for determination of carbofuraninvegetablesby high performance liquid chromatography (HPLC) with purification ofgel permeation chromatography (GPC). Methods Samples were extracted withacetonitrile and dissolved with ethyl acetate-cyclohexanemixture, and then separated by reversed-phase C18column followed with GPC purification.The fluorescence excitation and emission wavelengths were 285 nm and 320 nm. Identification of carbofuran was performed by HPLC with an external standard method. Results Carbofuranhad a good linear relationship in the range of 0.2~5.0 ng/mL with the correlation coefficient greater than 0.999. The limit of detection(LOD) was0.01mg/kg. The average recoverie