响应面法优化巴戟天低聚糖提取工艺 - 中国药房.pdf

响应面法优化巴戟天低聚糖提取工艺 1＊ 2 2 2 1# 1 1 杨 欣 ，宋健平 ，关业枝 ，王 琪 ，林 励 ，林伟栋 ，顾善喜 （1.广州中医药大学中药学院，广州 510006； 2.广州中医药大学科技产业园，广州 510445） 中图分类号 R284.2 文献标志码 A 文章编号 1001-0408（2015）34-4847-04 DOI 10.6039/j.issn. 1001-0408.2015.34.31 摘 要 目的：优化巴戟天低聚糖提取工艺。方法：以液料比、提取时间和提取温度为考察因素，以巴戟天低聚糖提取率为评价指 标，在单因素试验的基础上，采用3 因素3水平Box-behnken 中心组合试验，建立低聚糖提取得率的二次多项式回归方程，经响应 面回归分析得到优化组合条件并进行验证。结果：最优提取工艺条件为液料比23∶1（ml/g ）、提取时间1.7h 、提取温度93 ℃、提取 2次。该条件下3次验证试验低聚糖提取率实测均值为10.29％（RSD ＝0.20％，n ＝3），与预测值10.37％相比偏差为0.06％。结 论：采用响应面法可实现巴戟天低聚糖提取工艺的优化。 关键词 巴戟天；低聚糖；提取工艺；响应面法；优化 Optimization of the Extraction Technology for Oligosaccharides of Morinda officinalis by Response Surface Methodology 1 2 2 2 1 1 1 YANG Xin ，SONG Jian-ping ，GUAN Ye-zhi ，WANG Qi ，LIN Li ，LIN Wei-dong ，GU Shan-xi （College of Chi- nese Materia Medica ，Guangzhou University of TCM ，Guangzhou 510006，China ；2.Science Technology Indus- trial Park ，Guangzhou University of TCM ，Guangzhou 510445，China ） ABSTRACT OBJECTIVE ：To optimize the extraction technology of oligosaccharides of Morinda officinalis . METHODS ：The liq- uid/solid ratio ，extraction time and temperature were chosen as factors ，and the yield of oligosaccharides was estimated as index. On the basis of single-factor experiments ，using a 3-factor ，3-level Box-behnken central composite experimental design ，two-poly- nomial regression equation of extraction rate of oligosaccharide was established ，and it was analyzed by response surface methodolo- gy to obtain the optimum extraction conditions ，and the verification test was conducted. RESULTS ：The optimum extraction condi- tions were as follows as material/liquid ratio of 23∶1（ml/g ），extraction time of 1.7h ，and extraction temperature of 93 ℃，extract- ing for twice. Under these conditions ，the estimated and obs