转不同rol基因741杨株系相关性状比较分析 - 植物遗传资源学报.pdfVIP

植物遗传资源学报2010，11(4)：451—456 JournalofPlantGeneticResources 转不同rol基因741杨株系相关性状比较分析 王连荣1”，缪丽萍1，张晓军1，杨敏生1 (1河北农业大学林学院，保定071000；2河北北方学院园艺系，张家口075131) 杨株系生根率，叶绿素a、b含量。Pl值、ZT和IAA含量均高于转rolC基因741杨株系。 关键词：rol基因；74l杨；生长状况；叶绿素；叶绿素荧光；内源激素 ofRelatedTraits the Comparison amongTransgenic 741、订thDifferentrolGene Poplar WANG t2，MIAO Lian—ron91 Li．pin91，ZHANGXiao—janl，YANGMin—shen91 College，AgriculturalUniversityofHebei，Baodin9071001；2HorticultureCollege， (’Forestry HebeiNorth University，Zhangfiakou075131) this 741 withrolBandrolCweredetectedformoleculeandtheirroot Abstract：In study，thetransgenicpoplar fluorescence wellas hormones growthvariation，height，chlorophylla，b，chlorophyll characteristics，asendogenous and of 741 withrolBan— weredetermined．Theresultsshowedthattherootnumber rate transgenicpoplar rooting be— drolCwere thanthecontr01．The 741withroIB withno difference higher transgenicpoplar height significant tweenthe content values content control，chlorophylla，b increased，Fv／Fm，PIdecreased，IAA，ZT 1 rolBand ofthe contentdecreased．The 74with rolC：the weakened，dwarf transgenicpoplar topedge plants，chloro· contentwas values contentincreased．Overall a。b reduced，Fv／Fm，PIdecrease，IAA，ZT，A