用荧光标记mvr- pcr方法研究中国汉族人群 - 实验科学与技术.pdfVIP

　遗 传 学 报 　Acta Genetica Sinica , J anuary 2003 , 30 ( 1) : 15～19 ISSN 0379 - 4172 Studying the Polymorphism at DYF 155S 1 Locus in Chinese Han Population by MVRPCR Marked with Fluorescence ① YU Lei , WU XinYao , HUAN G YanMei , CAI GuiQing , XU ChuanChao , TON G DaYue ( Dep artment of Forensic Medicine , S un Yatsen Medical College , S un Yatsen University , Guangzhou 　510089 , China) Abstract : The simple and useful genotyping methods of D YF155S 1 locus by silver staining and fluorescence detection have been established. The blood samples from 155 unrelated males in Chinese Han population were typed by these two methods respectively and the same results were obtained. Among the 155 samples 66 alleles were found ,out of them 38 were observed once only. The most frequent alleles named 18 or 22 ,which frequency was 0065 ,the size of their first DNA fragments was 180 bp and the number of fragments was 16 or 17 . The gene diversity ( h) was 09789 . Out of the 155 samples ,25 samples had one or two bands lost (null repeat) among the successive bands. It was demonstrated by sequencing that the position of the lost bands corresponded to type 3 repeats. Our results indicated that the method ,which revealed the 5′end diversity of D YF155S 1 locus ,was a technique that could obtain the most Y - specific polymorphic information only by one PCR reaction . The allele frequencies of D YF155S 1 locus in Chi nese Han population provided the basic data for the study of population genetics and forensic practices. Key words : MVR - PCR ; MSY1 ( D YF155S 1) ; DNA polymorphism 用荧光标记 MVR - PCR 方法研究中国汉族人群