坛紫菜凝集素的糖结合专一性和细胞凝集作用 - 植物科学学报.pdfVIP

2002, 20(2) : 137140 J ournal of Wuha n Botanica l Resear ch 1 2 1 1 郑　怡 　余　萍　刘艳如　江红霞 ( 1. , 350007; 2. ; 350007) : , - -100 , DEAE Sepharose SephadexG ( PHL) 3 ( ) , , PHL ;PHL , PHL ,, : ; ; : 946: : 1000-470 (2002) 02-0137-04 A X - Specificity of Sugar binding and Cell Agglutination of Porp hy ra haitanensis Lectin 1 2 1 1 ZHENG Yi , YU Ping , LIU Yan-Ru , JIANG Hong-Xia ( 1.Bioengineering College, Fuj ian Teachers University, Fuzhou350007, China; 2.Institute of Polymer Sciences, Fuj ian Teachers University, Fuzhou350007, China) Abstract: The extraction of the thallus of Porp hyra haitanensis by phosphate buffer was precipi- tated with ( MH4 )2SO4 , purified further by DEAE-Sepharose column and Sephadex G-100 column. T he purified lectin (PHL) could bind monosaccharides (Ara, Gal and Xyl) and maltose to which PHL had the strongest binding. In cell agglutinations, PHL agglutinated erythrocytes from rabbit sheep and chicken but not from duck pigeon and human A B or O blood cell, , , , groups Furthermore PHL agglutinated marine microalga Pavlova v iridis and fresh microalga. , , , , Chlorella pyrenoidosa These agglutinations were related to algal cell density The result that. . Bacillus subtilis and Saccharomycs cerev isiae in different conditions responsed to PHL differently indicated that lectin receptors on cell cha