基因芯片技术（Gene chip technology）.docVIP

基因芯片技术（Gene chip technology） Collect and organize yourself Mistakes are inevitable For reference only If there are any mistakes Please correct me! thank you Gene chip technology Author: feng yongqiang yan xiaojun su cheng zhi Along with the gradual implementation of the Human genome project and the rapid development of molecular biology related disciplines More and more plants and animals and microbial genomes have been sequenced Genetic sequencing data is growing at an unprecedented rate However, how to study the function of so many genes in the life process becomes the common topic of life science workers around the world To do this It is very important to establish new methods of hybridization and sequencing to conduct efficient, rapid detection and analysis of a large number of genetic information Genetic chips (also known as DNA chips and biochips) are the products of this scientific development Its emergence provides a glorious prospect for solving such problems The technique refers to the hybridization of a large number of probe molecules (usually more than 400 per square centimetre) with the probe molecules attached to the supporting objects The number and sequence information of the sample molecules were obtained by detecting the cross-signal strength of each probe molecule Back in the 80s Bains W. Wait for [1] to fix short pieces of DNA to the support The sequence was determined by means of hybridization However, the industrialization of the gene chip from the laboratory is directly attributed to the combination of the probe solid phase in situ synthesis technology and photographic plate printing technology and the introduction of laser confocal microscopy It makes the synthesis, the fixed density of the tens of thousands of probe molecules practical and feasible And the laser confocal scanning technology enables real-time, sensitive and accurate detection and analysis of hybrid signals As is the case with the development of electronic tube circuits to tra