不同因素对家兔离体肠肌运动的影响 (1).docVIP

不同因素对家兔离体肠肌运动的影响 摘要 【目的】研究0.01%乙酰胆碱（Ach）、0.01%肾上腺素(E)、1当量NaOH、1当量HCl、23℃台氏液、缺氧等条件对家兔离体肠肌运动的影响。【方法】向浸有离体家兔肠肌的台氏液中依次滴加0.01% Ach、0.01%E、1N NaOH、1N HCl、38℃的台氏液迅速换成23℃的台氏液及停止供氧，并用生物信号采集处理系统记录肠肌的收缩情况。【结果】滴加0.01%Ach、1N NaOH 后，肠肌运动张力和频率都增大；滴加0.01%E、1N HCl 肠肌运动张力与频率迅速降低；将38℃的台氏液迅速换成23℃的台氏液后，肠肌运动肠肌运动张力与频率先迅速降低后逐渐增大；停止通氧后，肠肌运动张力和频率逐渐降低。【结论】一定浓度Ach、NaOH 可促进肠肌的运动；一定浓度的E、HCl 可以抑制肠肌的运动；短时低温能使肠肌运动稍降低后又加强直至正常；短时缺氧能使肠肌运动减弱。 关键词： 离体肠肌；台氏液；Ach；E；NaOH；HCl Abstract:【Objective】research 0.01% acetylcholine (Ach), 0.01% epinephrine (E), 1 equivalent NaOH, 1, 23 ℃ equivalent HCl in Taiwan's fluid, anoxia conditions from the body of rabbit intestinal muscle movement influence.【Method】a rabbit to dip vitro intestinal muscle in the Taiwan's liquid drop in turn add 0.01% Ach, 0.01% E, 1N 1N NaOH, 38, of HCl in Taiwan's liquid ℃ replaced the Taiwan's 23 ℃ liquid oxygen and stop, and biological signal acquisition and processing system records of intestinal muscle contraction.【Aesult】drops of 0.01% Ach, add NaOH 1N after intestinal muscle movement, tension and frequency increases; Drops of 0.01% E, add 1N intestinal muscle movement HCl in reduced quickly; and the frequency of tension 38 ℃ of Taiwan's replaced 23 ℃ liquid of Taiwan's liquid intestinal muscle movement after intestinal muscle movement tension and frequency after falling rapidly increased gradually first; Stop after the intestinal muscle movement,oxygen tension and frequency gradually reduced.【Conclusion】certain concentration Ach, intestinal muscle NaOH can promote the sports; Certain concentration can inhibit the E, HCl in intestinal muscle sport; Short-term low temperature can make slightly reduced intestinal muscle movement and strengthen until normal; Short-term hypoxia can make intestinal muscle motion decreases. Keyword: Intestinal muscle from the body; Taiwan's fluid; Ach; E;NaOH; HCl in 引言 肠道平滑肌有壁内神经丛，因此有自动节律性收缩的特性，其特性起源于肌肉自身并受中枢神经系统和体液因素的调节。平滑肌对某些化学物质（如：酸、碱等）及消化道神经释放的递质的刺激具有较强的敏感性。离体肠段虽然失去外来神经的支配，但肠壁神经丛仍存在，在适宜的条件下，仍能保持平滑肌收