狭基线纹香茶菜水溶性成分及其抗肿瘤活性研究.docVIP

狭基线纹香茶菜水溶性成分及其抗肿瘤活性研究 唐海明1，陈建南1，2，徐香琴1，赖小平1，2，黄 松1，2* （1.广州中医药大学新药开发研究中心，广东 广州510006； 2.东莞广州中医药大学中医药数理工程研究院，广东 东莞 523808） 摘要: 目的 研究狭基线纹香茶菜的水溶性成分及其抗肿瘤活性。方法 采用大孔树脂D101、ODS、Sephdex LH-20等色谱技术和重结晶进行分离纯化，通过ESI-MS、1H-NMR、13H-NMR等现代波谱技术进行结构鉴定；以HepG2为供试细胞株，采用MTT法对化合物进行体外抗肿瘤活性研究。结果 从狭基线纹香茶菜水提液中分离得到12个水溶性化合物，经波谱数据分析分别鉴定为Vitexin Ⅱ(1)， vicenin Ⅲ(2)，isoschaftoside(3)，schaftoside(4), vitexin(5)， 6,8-di-C-a-L-arabinosylapigenin (6)， apigenin-7-O-glucuronide (7)， apigenin 6-C-?-D-xylopyranosyl-8-C-a-L-arabinopyranoside(8)，apigenin 6-C-?-L-arabinopyranoside-8- C-a-L-arabinopyranoside (9)， caffeic acid(10)，rosmarinic(11)， rutin(12)。结论 化合物1～9为首次从香茶菜属植物中分离得到；其中化合物1、6、11对HepG2细胞有较好的抑制作用。 关键词：狭基线纹香茶菜；Vitexin Ⅱ；vicenin Ⅲ；isoschaftoside；schaftoside Water-soluble Chemical constituents with Anti-tumor Activities on HepG2 from Isodon Lophanthoides TANG Hai-ming1， LAI Xiao-ping1，2，1, Jian-nan1，2，HUANG Song1，2* 1.New Drug Research ＆ Development Center，Guangzhou University of Chinese Medicine，Guangzhou 510006，China2.Dongguan Institution for Mathematics and Theoretics Engineering Research，Guangzhou University of Chinese Medicine, Dongguan 523808，ChinaABSTRACT] Objective: To investigate water-soluble chemical constituents of Isodon Lophanthoides. Methods: Compouds were isolated and purified by D101, Polyamid eP, Sephadex LH-20 and ODS column chromatography. Their chemical structures were elucidated on the basis of spectral data and physicochemical properties. Results: Twelve compounds were isolated and identified as Vitexin Ⅱ(1), vicenin Ⅲ(2), isoschaftoside(3), schaftoside(4), vitexin(5), 6,8-di-C-a-L-arabinosylapigenin(6), apigenin-7-O-glucuronide (7), apigenin 6-C-?-D-xyl- opyranosyl-8-C-a-L-arabinopyranoside(8),apigenin 6-C-?-L-arabinopyranoside-8-C-a-L-arabi- nopyranoside (9), caffeic acid(10), rosmarinic(11), rutin(12). Conclusion: Compound 1-9 are separated from Isodon Lophanthoides for the first time, and constituents of the water-soluble parts of Isodon Lophanthoides were studie