神经生长因子控释凝胶促进大鼠周围神经损伤后再生的探究.pdfVIP

摘 要 目的：运用纤维蛋白胶(FG)结合神经生长因子(NGF)包裹SD大鼠坐骨神经断端， 通过纤维蛋白胶控释NGF以促进神经纤维再生并减少神经吻合口瘢痕形成。 方法：SD大鼠24只，随机分为2组，每组12只。A组：大鼠坐骨神经切断后 神经外膜直接缝合，然后吻合口包裹FG／NC,F凝胶，B组：神经外膜直接缝合，吻合 口未包裹凝胶。分别于术后第l、3、5、7周行术侧足迹实验检测，术后2月行术侧 三头肌湿重恢复率测量、电生理测定、甲苯胺蓝染色、透射电镜分析等。 结果：同一时间段两组间比较，各指标A组明显好于B组。 结论：NGF缓释凝胶可以促进大鼠坐骨神经离断损伤的修复并防止吻合口与肄围 组织粘连。 关键词：神经生长因子；纤维蛋白凝胶；周围神经；损伤；再生 of ofP NerveDefectwithControl StudyRepair eripheral in ReleasedNGFRats Abstract evaluatetheeffectofthecontrolledrelease Objective：To ofbiodegradable nervedefect．Methods： FG，nervegrowthfactor(FG／NGF)onperipheral ratswere dividedintotwo G／ towenty-fourSprague2Dawly randomly groups，F of B，n2 ratunderwent NGF(groupA，n：12)，Stitchingcombine(group12)．Each sciaticnerve intotwo thesedefectswere inthetwo right cuting pieces，then repaired different ofthe nerve was experimentalgroups．Theprocessperipheralregeneration evaluatedat2months theevaluateditemswere followingoperation,and footprinting test,giceps axon assessmentoftheoutcomeofnerve trihydmtemorphologic regeneration． Results：Nerve in Awas withthatseeninnatuai regenerationgroup comparable NerveandWas tothatinB inallindexes．Conclusion：： significantlysuperior groups controlledreleasewasa ideal，thatcan FG／NGF potential effectivelypromote nerve aftersciaticnervedefectofrets．TheeffectWasas ast