肾气丸剂量配比变化对肾损伤大鼠肾功能影响的探究.pdf

提 要 目的：研究肾气丸剂量配比变化对庆大霉素所致肾损伤大鼠肾功能及肾脏病理的 影响，探讨肾气丸剂量配比变化对药效的影响，以探求肾气丸最佳剂量配比。方法： 通过腹腔注射庆大霉素制造大鼠肾损伤模型，用不同剂量配比的肾气丸进行治疗，观 测大鼠血清尿素氮(BUN)、血清肌酐（Cr）及肾脏病理切片。结果：1、 造模过程中， 模型大鼠逐渐出现反应迟钝，自主活动减少，倦怠，精神萎靡，时扎堆，皮毛光泽度 差，竖毛，饮食减少，小便增多，大便质稀等症状。造模完成后，各组大鼠在用不同 剂量配比的肾气丸治疗过程中，以上症状逐渐好转2、血BUN、Cr及病理切片显示： 肾气丸剂量配比变化对庆大霉素肾损伤大鼠肾功能有不同的影响。结论：肾气丸中牡 丹皮、肉桂和附子的剂量变化对肾损伤大鼠血BUN、Cr的影响较大，肾气丸抗实验性 肾损伤的最佳药物配比为生地黄 8 份、山茱萸 4 份、山药 4 份、茯苓 3 份、泽泻 3 份、牡丹皮2份、肉桂2份、附子2份。 关键词 肾气丸；剂量配比变化；肾损伤；理论探讨；实验研究 Explore Changes in shenqi pill dose ratioof renal function in renal injury of rats Speciality: TCM clinic basis-JinGuiYaoLue Author:Zheng Xinggang Tutor:Prof.Tao Hanhua Abstract Objective: To study the influence of renal function and renal pathology of renal injury rats by gentamicin with the different shenqi pill dose pairing ，discuss the changes in kidney pill quantity influence of ratio of drug, in order to seek the the optimal dose ratio of kidney pills. Methods: Manufacturing renal injury rats model by intraperitoneal injection ， ， of gentamicin then treat with the different shenqi pill dose pairing observe the rats’ serum urea nitrogen (BUN), serum creatinine (Cr) and renal biopsy. Results: 1. During the model process, the rats appear burnout, spirit dispirited, crowded together, reduced activities, afraid of cold, no gloss of fur, clear and loose stool and other symptoms. After finishing the model, treat the groups of rats with shenqi pill, their above symptoms gradually improving. 2. BUN, Cr and renal biopsy observation display: the different shenqi pill dose pairing to kidney injury rats caused by gentamycin have different effects of renal function. Conclusion: The changes of