不同接毒方法对猪繁殖与呼吸综合征病毒增殖效果比较_吕永钢.pdfVIP

34 2010, 44( 3): 34~ 36/, 吕永钢, 吴 韬, 王朝蕾, 焦铁军, 崔卓宇, 杨鹏程, 王 昆 (, 712100) [] 2009- 12- 11 [] A [] 1002- 1280 ( 2010) 03- 0034- 03 [] S852. 651 [ ] 通过对猪繁殖与呼吸综合征病 ( PRRSV ) 感染M arc- 145细胞 4种不同接 方法的比 较研究, 筛选出一种在规模化生产中既能保证 价, 又能减少工艺流程, 提高工作效率, 降低污染几 率及生产成本的接种方法结果表明, 与其他方法相比, 同步接 法效果最佳, 不仅 价最高, 而且 繁殖 液时间缩短, 减轻了工作量, 节省生产成本 [] 猪繁殖与呼吸综合征病 ; M arc- 145细胞; 接 方法 Effect Comparison ofDifferent InoculationM ethods of PorcineReproductive andRespiratory SyndromeVirusMultiplication LV Y o g- ga g, WU Tao, WANG Zhao- lei, JIAO T ie- ju , CU I Zhuo- yu, YANG Pe g- che g, WANG Ku ( Yangl ing L vf ang B io - eng in eering Co. , L TD, Yang ling, Shaanx i 712100; China ) Abstract: Four differe t i oculatio methods of porci e reproductive a d resp iratory sy drom e virus ( PRRSV ) i fecti gM arc- 145 cellswere compared i PRRSV a tige flu id yield, a d a scie tific a d effective i ocu latio method fit for the i dustrial productio requ irem e ts w as scree ed ou,t it ca reduce the tech ology process, mi prove w ork efficie cy, decrease pollutio probab ility a d productio costs, e sure virus titers at the sam e tmi e. The resu lts show ed that them ethod of smi ulta eous i oculatio w ith PRRSV to the culture medium had the best effect compared w ith other methods. It e sured the h ighest virus titers a d ca shorte harvest tmi e, save raw materials, ease thew orkload. Key ords: PRRSV; M arc- 145 cells; i ocu latio m ethod [ 4] ( Porci e Reproductive PRRSV, a d Resp iratory Sy drome, PRRS) PRRSV , , , [ 5] , , ,