PLGA-PCL多聚物单丝生物可吸收缝线的临床前期 - 第三军医大学学报.DOC

PLGA-PCL多聚物单丝生物可吸收缝线的临床前期研究 陈翔宇，任小宝，刘明华，王 伟，王 涛，孙 溦 （400038 重庆，第三军医大学西南医院急救创伤中心）[摘要]目的聚乳酸-羟基乙酸共聚物poly(lactic-co-glycolic acid)-polycaprolactone，PLGA-PCL]多聚物单丝生物可吸收缝线的生物可吸收性、降解过程中的力学特性衰减、细胞毒性、组织相容性。方法hosphate buffered saline，PBS）或人血清溶液（human serum solution，HSS）中，观察其重量的衰减率；检测体外降解后其抗张强度的变化；与不同细胞系共培养后，检测细胞系的生长曲线变化；将不同可吸收缝线植入SD大鼠体内，观察局部炎症反应。结果PLGA-PCL多聚物单丝生物缝线olycaprolactone，PCL）的含量可很好的控制PLGA-PCL多聚物单丝生物缝线onofilament Bio-absorbable Sutures Chen Xiangyu，Ren Xiaobao，Liu Minghua，Wang Wei，Wang Tao，Sun Wei（400038 Chongqing， Trauma Center of SouthWest Hospital of The Third Military Medical University） [Abstracts] Objective To evaluate the absorbability，cytotoxicity， histocompatibility and break strength retention of complex PLGA-PCL monofilament bio-absorbable sutures. Methods The weight residual and break strength retention of those sutures were observed in vitro degradation tests. Than cell culture and animal mode were employed to evaluate their cytotoxicity and histocompatibility Results In PBS，the order of residual weight was chromic catgut S4 S3 S2 S1. In HHS，the order of residual weight was chromic catgut S3 S2 S4 S1. The morphology of complex polymer monofilaments，analyzed by SEM，showed some cracks appeared on surface after degradation. All monofilaments showed lower cytotoxicity and inflammatory response compare to chromic catgut. Conclusions Complex PLGA-PCL monofilament as potential bio-sutures showed expectable absorbability，cytotoxicity and histocompatibility. It’s possible that the absorbability and mechanical property retention of complex PLGA-PCL monofilament bio-sutures could be controllably changed by changing the ratio PCL within a certain range. [Key words] PLGA；PCL；Bio-suture Supported by the Tackling Project for Sci Tech Research Project of Chongqing（CSTC 2010AB5118）. Corresponding author: Ren Xiaobao, E-mail: rxb1000@ [基金项目] 重庆市科技攻关项目CSTC 2010AB5118） [通信作者] 任小宝，E-mail：rxb1000@ 缝线是外科手术中至关重要的材料之一，主要分为可吸收缝线和不可吸收缝线[1]。可吸收缝线由于其可降解性及良