重楼克感滴丸药效学效应探究.pdfVIP

提 要 目的：通过动物实验观察重楼克感滴丸治疗上呼吸道感染的药效学效应。 方法：研究重楼克感滴丸对镇咳、平喘、化痰、发汗、解热、抗炎和利尿、肠蠕 动的影响，小鼠吞噬细胞功能、血清溶血素、淋巴细胞Ｅ-花环数目的作用；采用牛 津杯法和双倍稀释法，测定滴丸对4种革兰阳性及3种革兰阴性细菌的体外抑菌作用。 结果：重楼小剂量组对小鼠咳嗽潜伏期及咳嗽次数影响明显（P0.05）；重楼大、 中剂量组可增强哮喘潜伏期（P0.05）；重楼大剂量组祛痰作用显著（P0.05），并能 增强小鼠足跖汗液分泌（P0.05）；重楼各组解热两实验都不具有明显作用（P﹥0.05）； 重楼克感滴丸可以加快大鼠回肠平滑肌蠕动。重楼大、中剂量组能加强小鼠吞噬细胞 功能（P0.05）；重楼大剂量组对细胞免疫作用显著（P0.05）；重楼各组均可降低血 清溶血素水平；重楼大、中剂量组能抑制小鼠耳肿胀（P0.05）；但重楼各组对大鼠 足肿胀抗炎不明显（P﹥0.05）；重楼小剂量组不仅减轻呼吸道炎症且利尿效果明显。 重楼克感滴丸在体外对金黄色葡萄球菌、乙型溶血性链球菌、大肠埃希菌和福氏志贺 氏菌具有良好体外抑菌作用。 结论：重楼克感滴丸具有发汗、抗炎、镇咳、平喘、化痰作用，可以增强机体的 非特异性免疫和细胞免疫，可加快肠平滑肌蠕动及轻度促进尿液排出，在体外可抑制 大肠埃希菌等。 关键词 重楼克感滴丸；药效学 The Experiment Study of Chongloukegan Dripping Pill on Major Pharmacodynamics in Animal Speciality:Basic research of integrated TCM and Western Medicine Author:Men yu-zhi Tutor: Wang shu-rong Abstract Objective: To explore the effect of Chongloukegan dripping pill on URTI by animal’s experiment. Method: To observe the antitussive,expectorant , antiasthma ,diaphoresis, antipyretic and anti-inflammatory effects of dripping pills; meanwhile to study enterokinesia and diuresis,at the same time to partly test mice’ blood serum hemolysin,lymphocytes’ E-rosettes and phagocytes; to determine on 4 kinds of Gram positive and 3 kinds of Gram negative bacteria by tube double dilution method and Oxford cup method. Results : The Chong-lou small dose could decrease the frequency of cough and prolong the latent period of cough in mice. The Chong-lou large dose and medium dose also could lengthen the asthma incubation period. The Chong-lou large dose could strengthen expelling phlegm and watered in voix pedis of mice. It was not obvious in the two kinds of experiment of relieved fever. The smooth muscle of ileum could be enhanced by dripping pills.The Chong-lou large