隔药灸脐法治疗原发性痛经的代谢组学探究.pdfVIP

提 要 目的：本研究采用隔药灸脐法治疗原发性痛经，以健康受试者为对照，观察治 疗前后血浆及尿液的代谢产物谱，找出潜在的生物标记物，推导相关代谢通路，借此 探明隔药灸脐法治疗原发性痛经效应机理所在。 方法：选取原发性痛经患者 10 例，正常对照组 10 例，通过检测患者血液、尿 液等的代谢产物谱，寻找其潜在的生物标记物，推导相关代谢通路。 结果：隔药灸脐法治疗原发性痛经的代谢产物谱发生了显著变化，其主要表现 为：皮质酮、羟脯氨酸、亮氨酸、脱氧皮质酮及苯甲酸、二氢神经鞘氨醇，这些代谢 物涉及到体内类固醇激素、一氧化氮、神经递质代谢及氨基酸代谢等过程。 结论：隔药灸脐法治疗原发性痛经的代谢组学机制主要是通过调整原发性痛经 患者异常的生殖内分泌激素水平，改善其神经、免疫、内分泌网络状况，从而减轻痛 经的疼痛程度并改善其伴随症状。 关键词 隔药灸脐法；原发性痛经；代谢组学 The Study of Metabonomics of Umbilical Moxibustion with Herbal Medicine on Primary Dysmenorrheal Syndrome Speciality: Acupuncture ,Moxibustion and Massage Author: Yu Xiaojing Tutor: Gao Shuzhong Abstract Objective: By detecting metabolic spectrum of patients` blood and urea, to find out potential biological marker and try to derive related metabolic pathway of umbilical moxibustion on primary dysmenorrheal syndrome, and to try to find out the mechanism of umbilical moxibustion on primary dysmenorrheal syndrome. Methods: 10 primary dysmenorrheal patients were randomly selected in treating group and 10 healthy female in control group. By detecting metabolic spectrum of patients` blood and urea, to find out potential biological marker and deduce related metabolic pathway of umbilical moxibustion on primary dysmenorrheal syndrome. Results: The metabolism spectrum of patients with primary dysmenorrheal syndrome had significantly changed. The substances changed were corticosterone, hydroxyproline, Leucineand Deoxycorticosterone, 2-Methylbenzyl benzoate, Pidolic acid, Ethyl homovanillate, p-Coumaric acid, and Piperidineand Sphinganine. These metabolites involved Steroid hormone biosynthesis, nitric oxide, neurotransmitters metabolism, and amino acid metabolism proc