隔药灸脐法治疗脾虚型肠易激综合征的代谢组学探究.pdfVIP

提 要 目的：本研究对脾虚型肠易激综合征患者进行隔药灸脐法治疗，通过检测治疗前 后患者尿样血浆的代谢产物谱，发现潜在的生物标记物，推导其相关代谢通路，籍此 探讨脐疗治疗该病可能的有效作用靶点，确定脐疗的疗效。 方法：选取 12 名脾虚型肠易激综合征患者，对治疗前、后该组患者尿样血浆进 行处理，根据 Simca-P 11.5 软件包的处理数据，通过 HMDB、METLIN 寻找有贡献的 代谢物组分，寻找相关代谢通路。 结果：隔药灸脐治疗肠易激综合征代谢谱发生了显著性变化, 其中变化较为显著 的是氨基酸、儿茶酚胺类、胆汁酸及内源性大麻素代谢物, 这些代谢物涉及到体内的 氨基酸代谢、神经递质代谢及内源性大麻素代谢等过程。 结论：脐疗可显著抑制肠易激综合征患者的内脏高敏性，改善肠道神经、免疫、 内分泌网络失调状况，改善脾虚型肠易激综合征患者的生存质量。 关键词 隔药灸脐法；神阙；脾虚型肠易激综合征；代谢组学 Metabonomics Study on Irritable Bowel Syndrome (IBS) of Spleen Deficiency Type by umbilical moxibustion Speciality: Acupuncture and Moxibustion Author: Wang Xiaocui Tutor:Gao Shuzhong Abstract Objective: The study on irritable bowel syndrome of spleen deficiency patients were treated with medicine-separated umbilical moxibustion. By detecting the patients urine and Plasma samples metabolite spectral before and after treatment, identify potential biomarkers and infer the related metabolic pathway. In order to discuss thepossible valid targets of umbilical moxibustion and determine the efficacy of umbilical moxibustion 。 Method: We selected 8 irritable bowel syndrome of spleen deficiency patients, deal with this group of patients urine and Plasma samples before and after treatment. According to the Simca-P 11.5software package processing data, look for the contribution of the metabolite fractions and search of related metabolic pathway by HMDB、KEGG. Results: Medicine-separated moxibustion at the navel treating irritable bowel metabolic profiling has undergone significant changes, including changes in more significant are amino acids, catecholamines, bile acids and endogenous cannabinoid metabolites, these metabolites involved in amino acid