富铬酵母对实验性糖尿病小鼠糖代谢酶活性的影响-第三军医大学学报.DOC

富铬酵母对实验性糖尿病小鼠糖代谢酶活性的影响 张宏馨1，张梅 1，王燕1，杨宏莉1，李军1，卢淑兰2，孙淑敏2 （1.河北大学医学部，保定，071000；2.河北省荣军医院，保定，071000） 摘要：目的：探讨富铬酵母对链脲佐菌素造模糖尿病小鼠糖代谢及关键酶己糖激酶、苹果酸脱氢酶活性的影响，探讨有机铬对糖尿病作用的机理和有效剂量。方法：用腹腔注射链脲佐菌素（STZ）的方法建立小鼠糖尿病模型，分别以高、中、低剂量富铬酵母和西药二甲双胍进行干预，实验期为4周，每周末尾静脉检测空腹血糖值，实验结束检测血糖值、己糖激酶（HK）、苹果酸脱氢酶活性（MDH）。结果：富铬酵母在125mg/kg剂量时显示降糖效果，在中、高剂量时与模型组对比有显著性差异。随富铬酵母剂量增高，对葡萄糖耐受能力增强。用药组肌肉组织HK、肝脏组织MDH活性与模型组对比有显著性差异。糖尿病小鼠血糖水平与HK、MDH活性呈显著的负相关性。结论：富铬酵母降低血糖的作用其机制之一可能是直接或间接提高了葡萄糖氧化分解的酶活性；富铬酵母对对抗糖尿病体重降低有一定作用。 关键词：铬；酵母；糖尿病；糖代谢；己糖激酶；苹果酸脱氢酶 Effect of the rich chromium yeast on sucrose metabolism enzyme activity of artificial diabetes mouse ZHANG Hong-xin1，ZHANG Mei1， WANG Yan1，YANG Hong-li1，LI Jun1，LU Shu-lan2，SUN Shu-min2（1.Hebei University Health Science Center,Baoding 071000，China;2. Rongjun Hospital of Hebei Province,Baoding 071000,China） Abstract: Objective: To explore the effect of chromium-enriched yeast on the glucose metabolism and the activity of key enzymes - hexokinase malic acid, dehydrogenase of Streptozotocin-induced diabetic mice; To study the Mechanism of Action and effective dosage in diabetes mellitus by organic chrome. Methods: Streptozotocin was injected intraperitoneally to 基金项目：河北省卫生厅项目（07325） 河北省科技计划项目-21） Supported by Health Department and Science and Technology Planning Project of Hebei（07325-21） 作者简介：张宏馨（1971-），女，硕士，讲师，主要从事药理药效方面的研究，发表专业论文9篇。 手机03123377472（小灵通）；E-mail：lzwen_@126.com establish diabetic model mice, to make intervention by low, middle, high dosage of chromium-enriched yeast and western medicine metformin. The experimental period was 4 weeks. Test the Fasting blood glucose each weekend at tail vein.. After the experiment, the blood glucose, hexokinase, malate dehydrogenase were tested. Results: it was showed that the dosage at 125mg/kg by chromium-enriched yeast has hypoglycemic effect. There was a significant difference as compared with model groups at middle and high dosage. The glucose tolerance enhanced with increase dosage