抄录（pdfファイル 365 mb）.pdf

A b s t r a c t I n v i t e d L e c t u r e I n v i t e d L e c t u r e Trafficking, cellular assembly and structure of collagen fibrils Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT UK KARL E. KADLER The goal of the Kadler laboratory is to understand how cells in tendons and ligaments build an extracellular matrix (ECM) comprising parallel arrays of collagen fibrils. Our research is directly relevant to statistics released by the NHS that 14,000 tendon and 85,000 ligament injuries are reported each year, with repetitive strain injury (RSI) alone costing UK industry up to ￡20 billion pounds per annum in lost working days. We are using high-resolution electron microscopy to determine the structure of the cell- ECM interface in tendons and ligament, the developmental biology of tendon and ligaments, and stem cell approaches to engineer these tissues in vitro and to devise novel therapies for tendon and ligament healing. Using serial section reconstruction we have identified novel plasma membrane protrusions, called fibripositors, in embryonic cells in tendon, ligament and other tissues where parallel collagen fibrils occur (Canty et al., 2004). Fibripositors contain collagen fibrils that extend from transport carriers within the cell to collagen fibril bundles in the ECM. Removal of fibripositor