番茄灰霉病拮抗细菌的筛选与x-75 菌株鉴定 - 园艺学报.pdfVIP

园 艺 学 报 2010 ，37 （2 ）：307 –312 Acta Horticulturae Sinica 番茄灰霉病拮抗细菌的筛选与 X-75 菌株鉴定 王 伟，李术娜，李红亚，郝志敏，王 全，王树香，朱宝成* （河北农业大学生命科学学院，河北保定 071001 ） 摘 要：采用琼脂平板扩散法筛选对番茄灰霉菌具有拮抗作用的细菌，经过对 200 株分离自土壤的细菌 菌株进行初筛、复筛后，得到了 1 株具有较高拮抗活性的菌株 X-75 。对该菌株进行形态观察和生理生化特征 分析，初步将其鉴定为芽孢杆菌属（Bacillus sp. ），将其 16S rDNA序列与GenBank 中已知标准菌株的16S rDNA 序列进行比对，并用 Neighbor-joining 方法构建 X-75 菌株进化树，结果表明，X-75 与标准菌株 AB245422 Bacillus velezensis 聚于同一分支，同源性最高，达 99.93%；利用番茄离体叶片对X-75 菌株的防效进行初步 检测，结果表明 X-75 菌株对番茄灰霉病菌具有明显的拮抗作用。 关键词：番茄；灰霉菌；芽孢杆菌；拮抗作用 中图分类号：S 641.2 文献标识码：A 文章编号：0513-353X(2010)02-0307-06 Screening of Antagonistic Bacteria Against Botrytis cinerea and Identification of Strain X-75 * WANG Wei, LI Shu-na, LI Hong-ya, HAO Zhi-min, WANG Quan, WANG Shu-xiang, and ZHU Bao-cheng (College of Life Science，Agricultural University of Hebei ，Baoding ，Hebei 071001，China) Abstract ：In order to obtain antagonistic bacteria against Botrytis cinerea, about 200 strains of bacteria isolated from soil samples were screened with the agar plate diffusion method. After primary and secondary screening, the strain X-75 showed the most significant antagonistic activity. After the morphological observation and a series of physiological and biochemistry experiments, the bacterial strain X-75 was primarily identified as Bacillus sp. The similarity of the 16S rDNA sequences between X-75 strain and AB245422 Bacillus velezensis was 99.93%, so it was finally identified as the nearest genetic relationship with Bacillus velezensis. And the X-75 strain showed obvious antagonistic activity against Botrytis cinerea Pers on tomato leaves. Key words：tomato ；Botrytis cinerea Pers ；Bacillus ；antagonistic activity 番茄灰霉病是由灰葡萄孢菌（Botrytis cinerea Pers ）侵染引起的病害，主要危害果实和叶子，引