利用弓形虫病人血清筛选弓形虫速殖子特异性抗原 - 中国药事.pdf

550 2011 25 6 * 张 影, 张 瑾, 杨英超, 薄淑英, 辛晓芳 , 王国治 ( , 100050) : 筛选能引起人体免疫反应的弓形虫抗原标记 利用实验室保存的9 份弓形虫病人血清 标准 6 份阴性血清标准 , 以及2 份弓形虫病人血清国际标准 作为 一抗, 分别与刚地弓形虫 (Toxoplasma gondii) RH 株的可溶性速殖子抗原 ( soluble tachyzoite antigen , STAg) 和排泄- 分泌抗 原 ( excreted/ secreted antigens , ESA) 进行免疫印迹反应 发现ST Ag 中有 10 条蛋白带与所用阳 性血清均发生免疫反应; ESA 中有4 条蛋白带能与所有阳性血清发生免疫反应, 这些蛋白与阴性血清均 不发生免疫反应其中分子量为3035495054kDa 的STAg 抗原以及分子量为23242 kDa 的 ESA 抗原能与所用阳性血清发生较强免疫反应 本实验结果将有助于寻找在弓形虫病人体内能够引 起免疫反应的弓形虫诊断抗原和免疫保护性抗原, 为弓形虫诊断试剂以及疫苗开发奠定初步基础 : 刚地弓形虫; 可溶性速殖子抗原; 排泄- 分泌抗原; 血清标准 ; 免疫印迹 : R3 233; R3 5 : A : ( 2011) Screening of Specific Antigen of Toxoplasma Gondii Tachyzoite by Using Human Serum Samples with Toxoplasmosis * Zhang Ying, Zhang Jin, Yang Yingchao, Bo Shuying, Xin Xiaofang and Wang Guozhi( National Institutes for Food and Drug Control, Beijing 100050) ABSTRACT Obj ective Screen antigenic markers of T . gondii tachyzoite evoking immune response in human. Methods Immunoblot w as conducted respectively for T . gondii soluble tachyzoite antigen ( ST Ag) and excreted/ secreted antigens( ESA ) against 17 human serum standards, w hich includes 9 human serum standard for AntiToxoplasma IgG and 6 negative serum standards of our laboratory , and 2 international human serum standard for AntiToxoplasma IgG. Results We detected 10 bands in STA g and 4 bands in ESA can react with all positive human serum, w hich w ere not reactive w ith negative human serum. The antigenic bands of apparent MW 30, 35, 49, 50, 54kDa of STA g and 23, 24, 2 kDa of ESA reacted distinctly w ith all human serum w ith toxoplasmosis. Conclusion T he results w ill help us to find diagnose marker and immunoprotection antigen w hich can cause immune response in human w ith